水溶性大豆多糖的分子表征和溶液流变学性质

Molecular Characterization of Soluble Soybean Polysaccharide and Rheological Properties of Its Aqueous Solution

采用核磁共振方法和凝胶渗透色谱(gel permeation chromatography,GPC)技术测定了水溶性大豆多糖(soluble soybean polysaccharides,SSPS)的酯化度和分子质量,并研究了SSPS在水溶液中的分子构象和流变学性质。结果表明,核磁共振方法可以快速准确地测定SSPS的酯化度,水溶液中SSPS呈紧密的无规线团构象,具有很低的本征黏度,SSPS水溶液表现出很强的剪切变稀行为,并具有明显的触变性。

The degree of esterification and molecular weight of soluble soybean polysaccharide（SSPS） were measured by 1H-NMR spectroscopy and gel permeation chromatography（GPC）, respectively, and the conformation of SSPS in water and the rheological properties of aqueous SSPS solution were investigated. It was found that the degree of esterification of SSPS could be determined by using 1H-NMR accurately and rapidly. The experimental results also showed that SSPS chains exhibited random coils with compact conformation in water, and that SSPS aqueous solutions possessed a very low intrinsic viscosity, significant shear thinning behavior and thixotropy.