MMP-2、MMP-14及VEGF-C在结肠癌组织中的表达及其临床意义

The expressions of MMP-2,MMP-14 and VEGF-C in colon cancer and their clinical significance

分析基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-14(MMP-14)及血管内皮生长因子-C(VEGF-C)在结肠癌中的表达及其与肿瘤临床特征的关系,探讨三者在结肠癌发生发展中的作用及临床意义。采用免疫组织化学方法检测30例结肠癌患者的癌组织及对应癌旁正常结肠组织中MMP-2、MMP-14及VEGF-C的表达情况,分析三者的表达水平与患者性别、年龄、组织学分级、Dukes分期、肿瘤大小、淋巴结转移等临床病理参数之间的相关性。结果显示:1 30例结肠癌组织中MMP-2、MMP-14及VEGF-C的阳性表达率分别为73.3%、80%和66.7%,而在癌旁正常结肠组织中的阳性表达率分别为10%、13.3%和16.7%,两组比较差异均有统计学意义(P<0.05)。2 MMP-2、MMP-14及VEGF-C的表达水平与肿瘤大小、Dukes分期和淋巴结转移有关(P<0.05),而与患者性别、年龄及组织学分级无明显相关性(P>0.05)。3MMP-2、MMP-14及VEGF-C三者两两间表达均相关(P<0.05)。4 30例结肠患者的癌组织中有16例共同高表达MMP-2、MMP-14及VEGF-C,与Dukes分期、病理分级和淋巴结转移有关(P<0.05)。

To investigate the role of MMP-2, MMP-14 and VEGF-C in colon cancer and its significance in colon cancer, we examine the expression of MMP-2, MMP-14 and VEGF-C by immunohistochemistry of 30 patients with colon carcinoma. The relationship between the expression of MMP-2, MMP-14 and VEGF-C and clinicopathological features including sex, age, tumor size, Dukes stage, pathological grade, and lymph node metastasis were examined. The positive rate of MMP-2, MMP-14, and VEGF-Cin the colon cancer tissues was 73.3%, 80%, and 66.7% respectively, while the positive rate of MMP-2, MMP-14, and VEGF-Cin the 30 normal colonic tissues were 10 %, 13.3 %, and 16.7 % respectively, and the differences were statistically significant（P〈0.05）. The expression of MMP-2, MMP-14, and VEGF-C related to tumor size, Dukes stages and lymph node metastasis（P〈0. 05）, but were unrelated to sex, age and histological grade（P〉0. 05）. Between MMP-2, MMP-14 and VEGF-C were related one to another （P〈0.05）. In 16 colon cancer specimens which showed high co-expression of MMP-2, MMP-14, and VEGF-C, the expression of these three factors were associated with Dukes stage, pathological grade and lymph node metastasis（P〈0.05）.