前列腺外周带基质细胞过表达LMO2基因对前列腺上皮细胞增殖能力的影响

Effect of prostate peripheral zones stromal cells on the proliferation of prostate cells by overexpression of LMO2 gene

目的观察前列腺外周带基质细胞过表达LMO2基因对前列腺上皮细胞增殖能力的影响。方法应用基因芯片筛选前列腺不同带基质细胞的差异基因。RT-PCR、Western印迹验证差异基因LMO2在基质细胞中的表达。CCK-8、EdU实验检测稳定表达LMO2的前列腺基质细胞株（WPMY-1-LMO2）条件培养基对BPH-1、PC3增殖能力的影响。细胞因子芯片检测WPMY-1-LMO2条件培养基中细胞因子的表达。Western印迹检测BPH-1、PC3细胞内增殖相关蛋白的变化。结果前列腺不同带基质细胞间有显著表达差异基因512条。RT-PCR、Western印迹证实LMO2基因在外周带基质细胞中过表达，外周带基质细胞中LMO2的表达水平比移行带基质细胞高3．36倍（2．89±0．33与0．86±0．10，P〈0．01）。WPMY-1-LMO2条件培养基孵育BPH-1（35．0％±2．5％）、PC3（42．7％±6．0％）细胞增殖能力与对照组（23．3％±2．5％、29．3％±3．5％）比较明显增强，细胞内STAT3磷酸化水平升高、CCND1表达增高。细胞因子芯片发现FGF-9及IL-11在WPMY-1-LMO2上清液中表达升高。结论前列腺外周带和移行带基质细胞中存在差异基因表达，前列腺基质细胞过表达LMO2后，诱导FGF-9、IL-11等细胞因子高表达，并通过旁分泌效应刺激前列腺上皮细胞生长。

Objective To explore the biological effect of prostate peripheral zones （PZs） stromal cells on the proliferation of prostate cells by overexpression of LMO2 gene. Methods Genes expressional distinction of different prostate stromal cells was screened by gene expression arrays. To validate the microarray data, real time-polymerase chain reaction （RT-PCR）, Western blotting analysis were used to check the over expression of LMO2 in PZs cells. To compare the effect of stromal cells which overexpressed LMO2 gene on in vitro proliferation ability of BPH-1 and PC3 cell lines, cell proliferation was measured by CCK-8 and EdU assay. Cytokines chip was used to screen expression of cytokines in WPMY-1-LMO2 conditioned medium. The changes of BPH-1 and PC3 proliferation associated proteins were assessed by Western blotting. Results A total of 512 genes were identified as markedly differentially expressed in stromal ceils originated from different zones. Among these genes, LMO2 gene was overexpression in peripheral zones stromal cells, and confirmed by RT-PCR and Western blotting,, Expression level of LMO2 gene was significantly up-regulated in peripheral zones stromal cells compared with transitional zones stromal cells, increased by 3. 36 folds on average （P 〈0. 01 ）. The proliferation of both PC3 and BPH-1 were found increased and STAT3 pbospborylation and CCND1 expression were increased after cultured in conditioned medium from stromal cells which stably expressed LMO2. Cytokines chip found increased FGF-9 and IL-11 expression in the medium supernatant reserved from LMO2-overexpressed stromal cell line. Conclusions Distinct gene expression exists among prostate stromal cells originated from different zones. LMO2 overexpressed stromal cells can induce prostate epithelial cell growth via paracrine of FGF-9, IL-11 or other cytokines.