超滤对身痛逐瘀汤镇痛抗炎效果的影响

Effects of Ultrafiltration Process on Analgesic and Anti-inflammatory Activity of Shentong Zhuyu Tang

目的:研究超滤对身痛逐瘀汤镇痛抗炎效果的影响,考察超滤技术用于该汤剂纯化的可行性。方法:以小鼠痛阈提高率,扭体潜伏期及15 min内出现的扭体次数、二甲苯致小鼠耳肿胀度为评价指标,考察超滤技术处理对身痛逐瘀汤镇痛抗炎效果的影响。结果:身痛逐瘀汤具有较好的镇痛抗炎效果,小鼠最大痛阈提高率(106.2±28.1)%,扭体潜伏期(355.1±42.8)s,15 min内出现的扭体数(30.8±7.7)次,二甲苯致小鼠耳肿胀抑制率41.27%,各指标与对照组相比均具显著性差异,20 nm和50 nm膜超滤液与未滤液相比镇痛抗炎效果无显著性差异。结论:超滤技术可用于身痛逐瘀汤的纯化工艺,超滤膜孔径应≥20 nm。

Objective： To investigate effects of ultrafiltration process on function of Shentong Zhuyu Tang and investigate feasibility of ultrafiltration technology for purification of this decoction. Method： Painful models were established with hot plate test or acetic acid writhing, inflammatory model were established with daubing dimethylbenzene on auricle. Pain threshold improvement rate of mice, latency of body-writhing,frequency of body-writhing response in 15 minutes and mice auricle swelling inhibition rate were used as indexes to observe effects of ultrafiltration process on analgesic and anti-inflammatory activity of Shentong Zhuyu Tang.Result： Analgesic and anti-inflammatory effects of Shentong Zhuyu Tang was remarkable,the largest percentage of pain threshold extension in mice rose to（106. 2 ± 28. 1） %, writhing incubation rose to（355. 1 ± 42. 8） s,number of writhing in 15 minutes rose to 30. 8 ± 7. 7,auricle swelling inhibitory rate rose to 41. 27%,significant difference in all indexes was seen as compared to the control group. No significant difference in all indexes was seen between groups of the non-ultrafiltration processed and ultrafiltration processed by 20,50 nm ultrafiltration membranes. Conclusion： Ultrafiltration technology can be applied to purify Shentong Zhuyu Tang, membrane pore size must be not smaller than 20 nm.