川归藿香颗粒挥发油提取工艺优选及药效学分析

Optimization of Extraction Technology of Volatile Oil in Chuangui Huoxiang Granules and Its Pharmacodynamics Analysis

目的:优选川归藿香颗粒中挥发油的提取工艺,并考察该制剂的药效。方法:以挥发油提取率为评价指标,采用正交试验考察萃取时间、压力、温度对川归藿香颗粒中挥发油提取工艺的影响,通过热板镇痛试验、扭体试验和耳肿胀试验考察颗粒剂的消炎镇痛作用。结果:最佳提取工艺条件为萃取压力25 MPa,萃取温度50℃,萃取时间1.5 h;挥发油提取率38.3mg·g^(-1)。按新、旧工艺制备的川归藿香颗粒具有镇痛、抗炎作用,且前者的药效强于后者。结论:优选的提取工艺设计合理、稳定可行,适合于工业化生产,制备的川归藿香颗粒能延长小鼠痛阈值,减少小鼠扭体次数,抑制小鼠耳肿胀度。

Objective： To optimize extraction technology of volatile oil in Chuangui Huoxiang granules and investigate its pharmacodynamics. Method： Taking yield of volatile oil as index,orthogonal test was adopted to optimize extraction technology of volatile components with extraction time,pressure and temperature as factors.Analgesic and anti-inflammatory effects of this granules was studied by hot plate analgesia experiment,writhing test and ear swelling experiment. Result： Optimum extraction conditions were as follows： extraction pressure of 25 MPa,temperature at 50 ℃ and lasting for 1. 5 h; yield of volatile oil was 3. 83 mg·g^-1. Chuangui Huoxiang granules had analgesic and anti-inflammatory effects which prepared by the new process or the old process,and efficacy of the former was stronger than the latter. Conclusion： This optimized extraction process is reasonable,stable and suitable for industrial production of Chuangui Huoxiang granules, this granules can prolong pain threshold of mice,decrease writhing response of mice and inhibit ear swelling degree in mice.