摘要
目的:优选小儿肺咳口服液的提取工艺,为其工业化生产提供参考。方法:通过镇咳、平喘、祛痰药效学试验优选小儿肺咳口服液的提取工艺路线。采用单因素试验考察加水量、浸泡时间、提取时间对干姜等药材中挥发油提取工艺的影响。以人参皂苷Rb1和橙皮苷含量、干膏得率为综合评价指标,采用正交试验考察加醇量、提取时间、提取次数对小儿肺咳口服液醇提工艺的影响。结果:最佳提取工艺路线为干姜等6味药材水蒸气蒸馏法提取挥发油,其余人参等16味药材进行醇提。挥发油最佳提取工艺为加8倍量水浸泡0.5 h,蒸馏8 h;挥发油提取量0.81 m L。最佳醇提工艺为加8倍量75%乙醇回流提取3次,每次2 h。人参皂苷类Rb1和橙皮苷提取量分别为1.59,2.84 mg·g^(-1),干膏得率19.53%。结论:优选的提取工艺稳定可行,适用于小儿肺咳口服液的工业化生产。
Objective: To optimize extraction process of Xiaoer Feike oral liquid and provide a reference for industrial production of this preparation. Method: Developing the antitussive,antiasthmatic and expectorant experiments,the best extraction method was optimized. Single factor tests was adopted to investigate effects of the amount of water, soaking time and extraction time on extraction process of volatile oil. With contents of ginsenoside Rb_1 and hesperidin, dry extract yield as comprehensive evaluation index, orthogonal design was adopted to predict ethanol process conditions by taking ethanol volume, extraction time and times as factors.Result: Optimum extraction process of volatile oil were as follows: adding 8 folds of water,extracting for 8 h after being soaked 0. 5 h; yield of volatile oil was 0. 81 mL. The best conditions for ethanol extraction process were extracting thrice with 8 times of 75% ethanol,2 hours for per time. Extracting amounts of ginsenoside Rb_1 and hesperidin were 1. 59,2. 84 mg·g^-1,dry extract rate was 19. 53%. Conclusion: These optimized technologies are stable and reliable for industrial production of Xiaoer Feike oral liquid.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第2期28-32,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
重庆市"121"科技支撑示范工程(cstc2014fazktjcsf10009)
关键词
人参皂苷RB1
橙皮苷
挥发油
镇咳试验
祛痰试验
平喘试验
小儿肺咳口服液
ginsenoside Rb1
hesperidin
volatile oil
antitussive test
expectorant test
antiasthmatic test
Xiaoer Feike oral liquid