清平片中黄酮类成分定量分析

Determination of Flavonoids in Qingping Tablet

目的:建立清平片中黄酮类成分槲皮素、葛根素、芦丁的含量测定方法,为清平片质量控制研究提供参考。方法:超声提取方法处理样品,采用Kromasil C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.05%三氟乙酸进行梯度洗脱,流速1.0 m L·min^(-1),柱温30℃,检测波长247 nm。结果:葛根素在0.003 6~0.114 0 g·L^(-1)(r=0.999 8),芦丁在0.003 3~0.105 0 g·L^(-1)(r=0.999 6),槲皮素在0.003 2~0.102 0 g·L^(-1)(r=0.9997)呈良好的线性关系,葛根素,芦丁,槲皮素平均回收率分别为99.60%,99.86%,99.94%,RSD分别为0.4%,0.3%,0.2%。结论:该方法快速、简便、重复性好,稳定,可控,可用于清平片黄酮类成分的含量测定。

Objective： To establish a method for the determination of quercetin,puerarin,and rutin in flavonoids of Qingping tablet,and provide a reference for the quality control of Qingping tablet. Method： The samples were extracted with ultrasonic extraction method. The Kromasil C_（18） column（4. 6 mm × 250 mm,5 μm）was used with a mobile phase of methyl alcohol-0. 05% TFA for gradient elution. The flow rate was 1. 0 m L·min^-1,with column temperature of 30 ℃ and the detection wavelength was 247 nm. Result： Puerarin,rutin and quercetin showed good linear relationship within the range of 0. 003 6-0. 114 0 g·L^-1（r = 0. 999 8）,0. 003 3-0. 105 0 g·L^-1（r = 0. 999 6） and 0. 003 2-0. 102 0 g·L^-1（r = 0. 999 7） respectively. The average recovery rate of three components was 99. 60% with RSD 0. 4%,99. 86% with RSD 0. 3% and 99. 94% with RSD 0. 2%respectively. Conclusion： The method is sensitive,simple,repeatable and controllable. It is a better method for determination of flavonoids content in Qingping tablet.