川牛膝化学拆分组分的制备及其免疫调节作用

Resolvability of Chemical Components of Cyathulae Radix and Their Immune Regulation Effects

目的:制备川牛膝化学拆分组分,并研究川牛膝水煎液及其各化学拆分组分对免疫调节的影响。方法:采用水煎煮、醇沉、大孔吸附树脂柱色谱分离技术的联合应用,将川牛膝水煎液的化学成分拆分为成分之间互不交叉的以下几部分:多糖组分,20%乙醇洗脱组分,甾酮组分和脂肪油组分,对其进行化学特征研究并运用HPLC对各化学拆分组分进行互不交叉性研究。考察川牛膝水煎液及其各化学拆分组分对小鼠碳粒廓清指数和免疫器官的影响及对小鼠血清溶血素抗体生成的影响。结果:建立的川牛膝化学成分拆分方法可行、稳定且重复性好,能够实现全成分拆分,各拆分组分间化学成分基本无交叉;川牛膝具有一定的免疫增强作用。结论:发挥免疫增强作用的药效物质基础为多糖组分和甾酮组分。

Objective： Preparating resolvability of chemical components of Cyathulae Radix and investigating water decoction and its chemical detached composition of Cyathulae Radix which impact on immune regulation. Method： We adopted the technology of hot water extraction,alcohol sedimentation and macroporous adsorptive resins to establish the split method of chemical components of Cyathulae Radix and four compositions obtained were fatty oils composition,polysaccharide composition,ketosteroid composition and 20% ethanol elution composition,repectively. We adopting the technology of high performance liquid chromatography to study their chemical property and the chemical composition was detached uncross in each composition. Investigating water decoction and its chemical detached composition of Cyathulae Radix which impact the carbon clearance index and the influence of immune organs in mice and in mice serum hemolysin antibody generatin on immune regulation.Result： The results indicated that establishing the detached method based on the chemical components of Cyathulae Radix was practicable,steady and good reproducibility. The characteristic of this method was holocomposition which was detached uncross in each. Cyathulae Radix had the certain effect of immunoenhancement.Conclusion： The effect of immunoenhancement of Cyathulae Radix which was based on polysaccharide and ketosteroid composition.