乳液电纺纤维膜固定化漆酶及其对水中酚类污染物的降解

Immobilization of laccase in electrospun fibrous membranes by emulsion electrospinning and its degradation of phenolic pollutants in water

为了提高漆酶在水体有机污染物控制中的实用性,以乳液电纺作为酶固定化方法,以电纺纤维膜作为载体制备固定化漆酶,考察并优化了纺丝电压、乳化剂和酶溶液添加量等因素对载漆酶电纺纤维膜形貌和酶学性能的影响。结果表明,在纺丝电压为12 k V、乳化剂F108添加量为10 wt%(相对于聚合物质量),漆酶溶液添加量为0.5 m L的最优条件下,所得电纺纤维膜固定化漆酶能保持78.6%的活性,且纤维呈壳-核结构,漆酶可以被成功包埋固定在电纺纤维的核部分,使漆酶具有良好的储存和操作稳定性。将固定化漆酶用于净化水中典型酚类污染物,结果表明,其对水中2,4-二氯酚、双酚A和三氯生的去除效率均高于75%;且得益于纤维膜对水中三氯生的强吸附作用,固定化漆酶对三氯生的降解率达到83.2%,甚至高于游离漆酶(74.3%)。乳液电纺纤维膜固定化漆酶在水中酚类污染物净化方面表现出良好的应用前景。

Laccase was immobilized in electrospun fibrous membranes through emulsion electrospinning to improve its practicability in controlling organic pollutants in water. Some parameters,including spinning voltage,the dosages of emulsifier and laccase solution,were optimized,and their effects on the morphology and enzymatic properties of laccase-carrying electrospun fibrous membranes（ LCEFMs） were investigated. The experimental results showed that,under the optimum conditions： spinning voltage was 12 k V,the dosage of emulsifier F108 dosage was 10 wt%（ relative to the poly（ D,L-lactide））,the volume of laccase solution was 0. 5 m L,the obtained LCEFMs could keep 78. 6% of initial activity. The fibers showed shell-core structure,and laccase could be successfully encapsulated in the core of electrospun fibers,which benefits the enhancement of storage and operational stability. The LCEFMs was used to remove typical phenolic pollutants from water,and the results indicated that the removal efficiencies of 2,4-dichlorophenol,bisphenol A and triclosan by LCEFMs were higher than 75%. Attribute to the strong adsorption of triclosan by electrospun fibrous membrane,the degradation efficiency of triclosan by immobilized laccase reached 83. 2%,which was even higher than that by free laccase（ 74. 3%）. Immobilization of laccase in electrospun fibrous membranes by emulsion electrospinning has a good prospect in the removal of phenolic pollutants from water.