养肺滋阴汤对干燥综合征小鼠模型干预作用

Intervention of Yangfei Ziyin Decoction on Sjgren′s Syndrome Model Mice

目的观察养肺滋阴汤对干燥综合征(Sjgren′s syndrome,SS)小鼠模型症状、血清TNF-α、IL-6、水通道蛋白-5(aquaporin-5,AQP-5)及病理学的影响。方法 60只小鼠按随机数字表分成模型组、正常组、养肺滋阴汤(中药)高剂量组、中剂量组、低剂量组(36.7、18.4、9.2g/kg,0.2mL/10g)及白芍总苷(白芍)组(0.6g/kg),每组10只。各组每周连续干预6日,停药1日,共50日。实验第10、20、30、40、50日测量各组小鼠体重、唾液分泌量、进食量、饮水量,并于第50日取血,摘取小鼠颌下腺、胸腺、脾脏称重,采用ELISA法检测血清TNF-α、IL-6、AQP-5含量,并观察颌下腺组织病理学变化。结果与正常组比较,模型组小鼠饮水量无变化,唾液分泌量减少(P<0.01,P<0.05)。模型组胸腺、脾脏、颌下腺重量及指数升高(P<0.01,P<0.05)。与模型组同期比较,白芍组、中药各剂量组唾液分泌量均增加(P<0.01,P<0.05)。与模型组比较,白芍组胸腺、脾脏、颌下腺重量及指数降低(P<0.01,P<0.05);中药低剂量组胸腺、颌下腺重量及指数降低(P<0.01,P<0.05);中药中、高剂量组胸腺、颌下腺重量及指数、脾脏指数降低(P<0.01,P<0.05);白芍组TNF-α、IL-6降低,AQP-5升高(P<0.05);中药中、高剂量组AQP-5升高(P<0.01,P<0.05)。模型组颌下腺组织内腺泡及导管破坏,腺泡及导管不规则,上皮变性坏死脱落,周围有轻至中度淋巴细胞浸润,中药各剂量组及白芍总苷组病理破坏减轻。结论养肺滋阴汤可改善SS模型小鼠症状、血清TNF-α、IL-6、AQP-5及病理学改变。

Objective To observe the effect of Yangfei Ziyin Decoction（YZD）on symptoms,serum levels of TNF-α,IL-6,and aquaporin-5（AQP-5）,and pathology of Sjgren′s syndrome（SS）model mice.Methods Totally60 mice were divided into 6 groups according to random digit table,i.e.,the model group,the normal control group,the high,medium,low dose YZD groups（administered with YZD at 36.7,18.4,9.2 g/kg,0.2 mL/10 g）,the Chinese patent medicine group[CPM,administered with total glucosides of paeony at 0.6 g/kg],10 mice in each group.All intervention was performed for six successive days in a week,with an interval of one day,a total of 50 days.Body weight,salivary secretion,food and water intake were measured at day10,20,30,40,and50,respectively.At day50 blood was collected.Submandibular gland,thymus,and spleen were weighed.Serum levels of TNF-α,IL-6,and AQP-5 were detected by ELISA.Pathological changes of submandibular gland were observed.Results Compared with the normal control group,there was no change in water intake of mice in the model group,but with reduced salivary secretion（P〈0.01,P〈0.05）.Thymus/spleen/submandibular gland weight and index increased in the model group（P〈0.01,P〈0.05）.Compared with the model group at the same time point,salivary secretion increased in the CPM group and3 YZD groups（P〈0.01,P〈0.05）.Compared with the model group,thymus/spleen/submandibular gland weight and index decreased in the CPM group（P〈0.01,P〈0.05）.Thymus/submandibular gland weight and index decreased in the low dose YZD group（P〈0.01,P〈0.05）.Thymus/submandibular gland weight and in-dex,and spleen index decreased in high and medium dose YZD groups（P〈0.01,P〈0.05）.Levels of TNF-αand IL-6 decreased,but AQP-5 level increased in the CPM group（P〈0.05）.AQP-5 level increased in high and medium dose YZD groups（P〈0.01,P〈0.05）.In the model group alveoli and duct of salivary gland were destroyed,alveoli and duct were irregular,epithelial cells were degenerated,necrotic,and desquamated.Mild-to-moderate lymphocytic infiltration occurred around submandibular gland.Pathological changes were alleviated in the CPM group and 3 YZD groups.Conclusion YZD could improve clinical symptoms,serum levels of TNF-α,IL-6,AQP-5,and pathological changes of SS model mice.