温阳汤对职业性哮喘模型大鼠CD34^+分化的影响

Effect of Wenyang Decoction on the Differentiation of CD34^+ Progenitor Cells in Occupational Asthma Model Rats

目的研究温阳汤对职业性哮喘模型大鼠CD34^+分化的影响。方法选择健康雄性SD大鼠50只,随机分为模型组、空白组、中药组、西药组、结合组,每组10只,其中西药组及中药组分别予泼尼松混悬液(10mg/kg)、温阳汤(20mg/kg)灌胃;结合组予温阳汤加泼尼松混悬液灌胃;空白组及模型组予生理盐水灌胃。每日1次,共14日。观察模型大鼠一般情况,采用ELISA法检测外周血IL-5、嗜酸细胞活化趋化因子(eotaxin)含量,采用瑞氏-吉姆萨染色计数骨髓悬液嗜酸粒细胞(eosinophils,EOS),采用流式细胞术计数CD34^+、嗜酸性粒细胞趋化因子受体(CC chemokine receptor 3,CCR3^+)。结果与空白组比较,模型组大鼠外周血IL-5、eotaxin表达升高(P<0.01),EOS、CD34^+细胞计数及CD34^+/CCR3^+升高(P<0.01)。与模型组比较,各给药组IL-5、eotaxin、EOS、CD34^+细胞计数及CD34^+/CCR3^+降低(P<0.01)。与西药组比较,结合组EOS细胞计数及CD34^+/CCR3^+降低(P<0.01)。与中药组比较,结合组EOS细胞计数降低(P<0.01)。结论温阳汤可以降低职业性哮喘大鼠体内炎症因子水平,抑制EOS的趋化、募集,从而减少CD34^+祖细胞向EOS分化。

Objective To study the effect of Wenyang Decoction（WD）on the differentiation of CD34^＋ progenitor cells of occupational asthma（OA）model rats.Methods Fifty healthy male SD rats were randomly divided into five groups,i.e.,the model group,the blank control group,the WD group,the Western medicine group,the combined group,10 in each group.Prednisone suspension（10 mg/kg）was administered to rats in the Western medicine group by gastrogavage.WD（20 g/kg）was administered to rats in the WD group by gastrogavage.Prednisone suspension plus WD was administered to rats in the combined group by gastrogavage.Normal saline was administered to rats in the model group and the blank control group by gastrogavage.The general condition of rats was observed.Expression levels of peripheral blood IL-5 and eotaxin,eosinophils（EOS）,CD34^＋ ,CC chemokine receptor 3（CCR3^＋ ）in bone marrow suspension were detected by ELISA,Wirght-Giemsa,and flow cytometry,respectively.Results Compared with the blank control group,expression levels of IL-5 and eotaxin in peripheral blood were significantly higher（P〈0.01）,and the count of EOS and CD34^＋ cells,as well as CD34^＋ /CCR3^＋ significantly increased（P〈0.01）in the model group.Compared with the model group,expression levels of IL- 5 and eotaxin,the count of EOS,CD34^＋ cells,CD34^＋ /CCR3^＋ were lowered in three treated groups（P〈0.01）.Compared with the Western medicine group,the count of EOS and CD34^＋ /CCR3^＋ decreased in the combined group（P〈0.01）.The count of EOS was significantlylower in the combined group than in the WD group（P〈0.01）.Conclusion WD could reduce levels of in vivoinflammatory factors,and restrain the differentiation and recruitment of EOS,thereby alleviating the differentiation of CD34^＋ progenitor cells to EOS.