摘要
本试验旨在探究高致病性蓝耳病(HP-PRRS)自然病例中致炎因子IL-1、IL-6和TNF-α含量,进而阐述HP-PRRS的致病机理,为养殖生产中的HP-PRRS防控提供理论基础。在成都地区4个发病猪场采集具有临床症状的猪血清(含5头濒死猪)35份与无临床症状猪血清23份,利用RT-PCR分别检测出HP-PRRSV阳性血清25份和11份。将检测出阳性表现症状的25份血清、检测阳性无临床症状的11份血清及检测阴性的12份血清分成3组,分别标注为病猪组、隐性感染组和健康对照组,运用ELISA方法进行IL-1、IL-6和TNF-α含量测定。结果显示,IL-1含量在三组中差异极显著(P<0.01),病猪组极显著高于隐性感染组与健康组(P<0.01),隐性感染组与健康对照组差异不显著(P>0.05);IL-6含量在三组中差异显著(P<0.05),病猪组显著高于隐性感染组与健康对照组(P<0.05),隐性感染组与健康对照组差异不显著(P>0.05);TNF-α含量在三组之间无显著差异(P>0.05)。该结果表明IL-1与IL-6在HP-PRRS感染致病中具有重要作用。
To find the relationship among IL-1、IL-6、TNF-α content in blood sera of HP-PRRSV infected pigs,so to explain the pathogenesis of HP-PRRS,and to provide a theoretical basis for the prevention and control of HP-PRRS,48 blood sera samples from 4 farms in Chengdu were sampled and tested by ELISA,including 25 RT-PCR positive samples with clinical symptoms and 11 positive samples without clinical symptoms and 12 negative samples.The results showed that the difference of IL-1 in positive pigs was significant（P〈0.01）compared with the other two groups,and the differences of IL-1 between the other two groups were not significant（P〉0.01）.The differences of IL-6 levels among the three groups were significant（P〈0.05）,and IL-6 in positive samples with clinical symptoms were higher（P〈0.05）than the other two groups.The differences of of IL-6 in the other two groups with negative results were not significant（P〉0.05）.The differences of TNF-α among the three groups were not significant（P〉0.05）.These results showed that IL-1and IL-6 increased in blood serum when pigs were infected by HP-PRRSV and played important role in infection,while TNF-α has little change.
出处
《中国动物检疫》
CAS
2016年第1期67-70,共4页
China Animal Health Inspection
