血清中MMP-9、TIMP-1表达水平与肺癌病理特征的关系分析

Analysis of Relationship between MMP-9 and TIMP-1 Expression in Serum and Pathological Features of Patients with Lung Cancer

目的检测肺癌患者血清MMP-9、TIMP-1水平变化,探讨其与肺癌患者病理特征的关系。方法选取80例肺癌患者为研究对象,另选取30例正常健康者为对照组,ELISA法测定肺癌患者及正常者血清中MMP-9、TIMP-1水平,比较两组之间差异。结果肺癌患者血清MMP-9、TIMP-1水平均显著高于正常者(P<0.01),肺癌患者血清中MMP-9、TIMP-1表达水平与肺癌病理类型、病理分化程度无关(P>0.05),与肿瘤体积大小、TNM分期、淋巴结转移、远处转移相关,肿瘤≥3 cm患者血清中MMP-9、TIMP-1水平明显高于肿瘤≤3 cm的患者;Ⅲ+Ⅳ期患者血清中MMP-9、TIMP-1水平明显高于Ⅰ+Ⅱ期患者;淋巴结转移患者血清中MMP-9、TIMP-1水平明显高于无淋巴结转移患者;远处转移患者血清中MMP-9、TIMP-1水平明显高于无远处转移患者;肺癌患者MMP-9表达与TIMP-1表达呈正相关(γ=0.634,P<0.05)。结论 MMP-9、TIMP-1在肺癌患者中表达与TNM分期、淋巴结转移、远处转移相关,在肺癌侵袭转移中有重要作用,可监测病情发展。

Objective To observe the change of MMP-9 and TIMP-1 expression in serum of patients with lung cancer,and to explore its relationship with the pathological features. Methods 80 cases of lung cancer patients were selected as the study group,and 30 cases of normal healthy patients were selected as the control group,MMP-9 and TIMP--1 levels in serum of lung cancer patients were detected by ELISA assay,the difference between the 2 groups were compared. Results MMP-9,TIMP-1 levels of patients with lung cancer were significantly higher than those of normal patients（ P〈0. 01）,MMP-9 and TIMP-1 expression had no relation with pathological type,pathological differentiation degree（ P〉0. 05）,but was related to tumor size,TNM stage,lymph node metastasis,distant metastasis. MMP-9 and TIMP-1 levels of patients with tumor ≥3 cm serum were significantly higher than patients with tumor ≤3 cm; MMP-9 and TIMP-1 levels of stage Ⅲand Ⅳpatients were significantly higher than stage Ⅰand Ⅱpatients; MMP-9 and TIMP-1 levels in patients with lymph node metastasis were significantly higher than those without lymph node metastasis; MMP-9 and TIMP-1 levels in patients with distant metastases were significantly higher than those without distant metastasis. Conclusion MMP-9 and TIMP-1 expression in lung cancer patients is related to TNM stage,lymph node metastasis and distant metastasis,it plays an important role in cancer invasion and metastasis,and it can monitor disease progression.