基于渗透调解物质及叶绿素分析红砂抗旱优良家系的早期选择

Early selection of drought-resistant superior families of Reaumuria soongorica based on the analysis of osmoregulation substances and chlorophyll

对生长健壮、无病虫害的77个3年生红砂家系进行可溶性蛋白(SP)、可溶性糖(SS)、脯氨酸(Pro)及叶绿素(Chl)含量测定,运用主成分分析法和隶属函数法进行抗旱性综合评价,筛选抗旱优良家系.结果表明:77个家系的叶片渗透调解物和叶绿素含量存在较大差异,可溶性蛋白含量为2.14~8.60 mg·g^(-1)FM,可溶性糖含量为6.82~21.86 mg·g^(-1)FM,脯氨酸含量为118.73~1494.30μg·g^(-1)FM,叶绿素a含量为321.88~897.37μg·g^(-1)FM,叶绿素b含量为53.65~249.04μg·g^(-1)FM,叶绿素(a+b)含量为387.39~1146.40μg·g^(-1)FM,叶绿素a/b值为3.46~6.42.各抗旱指标在红砂家系中差异显著,脯氨酸含量变异最大,可溶性糖含量次之.采用多指标的综合评价方法发现,12个家系为抗旱性优良家系,其中扎子沟1-2号和扎子沟1-1号家系表现最好.

By using 77 families of 3 year-old Reaumuria soongorica seedlings that grew well without infection of any pests and diseases as experimental material,the contents of soluble protein（SP）,soluble sugar（SS）,proline（Pro） and chlorophyll（Chl） were measured,and their drought resistance was evaluated with principal component analysis and subordinate function method.The results showed that there were great differences in leaf osmolytes and chlorophyll among the 77 families.The soluble protein content varied from 2.14 to 8.60 mg·g^-1 FM,the soluble sugar content was from 6.82 to 21.86 mg·g^-1 FM,the proline content was from 118.73 to 1494.30 μg·g^-1 FM,the chlorophyll a content was from 321.88 to 897.37 μg·g^-1 FM,the chlorophyll b content was from53.65 to 249.04 μg·g^-1 FM,chlorophyll（a＋b） was from 387.39 to 1146.40 μg·g^-1 FM,and the chlorophyll a/b was from 3.46 to 6.42.All drought-resistant indices had significant difference among R.soongorica families,among which the proline content varied most,followed by the soluble sugar content.Evaluated by using the synthesized multi-index,it was found that 12 families showed good drought resistance,with Zhazigou 1-2 and Zhazigou 1-1 performing the best.