成都地区2009—2013年儿童人博卡病毒感染分析

Prevalence of human bocavirus among child inpatients in Chengdu city,2009-2013

目的了解四川成都地区急性呼吸道感染住院患儿人博卡病毒（HBoV）的感染情况，分析其流行特征。方法采集2009年10月q013年10月年成都市某妇女儿童医院因呼吸道感染而住院的患儿鼻咽抽提物，提取核酸，用巢式PCR方法检测人博卡病毒；同时对流感病毒等其他7种呼吸道病毒进行检测，了解混合感染情况；用统计学软件对检测结果及病例信息进行分析。结果在1842份标本中，HBoV检出率为6．84％（126／1842），与其他呼吸道病毒混合感染率为58．73％（74／126）；患儿以下呼吸道感染为主，1—2岁检出率最高，为7．83％（108／1379）；HBoV感染未见明显的季节性分布特点。结论HBoV为成都地区儿童发热呼吸道症候群的主要病原之一，〉2岁儿童检出率最高，且与其他病毒有较高的混合感染率。

Objective To study the prevalence of human bocavirus （HBoV） among child inpatients with acute re- spiratory tract infection（ARTI） in Chengdu city and to analyze its epidemic characteristics. Methods Nasopharyngeal aspirates were collected from hospitalized children with ARTI from October 2009 to October 2013. HBoV capsid protein VP gene fragments were detected using nested-polymerase chain reaction（ nested-PCR）, while other 7 respiratory viruses were examined with nested-PCR simultaneously. Case information were also gathered for statistical analysis. Results Out of 1 842 specimens, 126（6. 84% ） were positive for HBoV and the rate of co-infection of HBoV and other respiratory viruses was 58.73% （74/126）in positive samples. The majority of infected children were under 2 years old, with a posi- tive rate of 7.83% （ 108/1 379 ）. HBoV infection showed no obvious seasonal variation. Conclusion HBoV is one of the main pathogens in children with febrile respiratory syndrome in Chengdu region, which is more prevalent in the chil- dren younger than two years old and is of higher co-infection rates with other respiratory viruses.