黄瓜香水提取物对四氯化碳诱导大鼠肝纤维化的影响

The Effect of VDG on Hepatic Fibrosis by Carbon Tetrachloride in Rats

探讨黄瓜香对肝纤维化大鼠肝组织α-平滑肌肌动蛋白(α-SMA)及生长转化因子β1(TGF-β1)表达的影响。将48只大鼠随机分为正常对照组、模型组、生理盐水组和黄瓜香干预组,分析仪检测血清ALT、AST、ALP、HA、LN、PCⅢ型胶原,HE染色显示肝的显微结构,SABC法免疫组织学染色显示α-SMA及TGF-β1。结果表明:与模型组和生理盐水组比较,黄瓜香组ALT、AST、ALP、HA、LN、PCⅢ型胶原含量均明显降低(P<0.01);黄瓜香干预组有较正常肝小叶结构,少见纤细的纤维间隔,有炎性细胞少量浸润;模型组、正常对照和黄瓜香干预组α-SMA及TGF-β1表达的平均灰度值比较均有显著性差异(P<0.01)。黄瓜香可明显减少纤维化组织面积,其在治疗肝纤维化方面有开发价值。

The effects of VDG on the expression of smooth muscle actin alpha （et-SMA） and transforming growth factor-β1 （TGF-β1 ） in rats with hepatic fibrosis were studied. Forty-eight rats were randomly divided into four groups： normal control group, model control group, normal sodium group and interrupt group. The contents of ALT, AST, ALP, HA, LN and PC m were measured by full automatic biochemical analyzer and ELISA, hepatic microstructure was showed by HE staining, the expression of α- SMA and TGF-β1 was showed by immunohistochemistry dyeing. Compared with the model group, the contents of ALT, AST, ALP, HA, LN and PC Ⅲ remarkably decreased （P〈0.01）. In interrupt group, the hepatic lobe structure was normal, the slender textile fiber gap was minority, and there was the few inflammatory cell infiltration. There was significant difference in the average grey degree of α-SMA and TGF-β1 among normal control group, model control group and interrupt group （P〈0.01）. The alcohol extract from VDG showed the protective effects for liver fibrosis and could alleviate the degree of degeneration and necrosis induced by CCl4.