高效液相色谱荧光法同时检测黄瓜及土壤中的阿维菌素和甲维盐

Multi-Residues Determination of Abamectin and Emamectin Benzoate in Cucumber and Soil by HPLC-FLD

利用高效液相色谱荧光法建立了阿维菌素和甲维盐在黄瓜中的多残留分析方法,样品用乙腈提取,直接离心净化后旋蒸浓缩,经三氟乙酸酐（TFAA）和N-甲基咪唑（NMIM）衍生化,过HPLC-FLD检测,结果显示：阿维菌素和甲维盐在0.01~2.0 mg/L标液范围内检测浓度与检测器响应峰面积呈良好线性关系,阿维菌素R2=0.9998,甲维盐R2=0.9993。在浓度为0.01~1.0 mg/kg的添加范围内,阿维菌素在黄瓜和土壤中平均回收率为87.12%~101.13%,RSD 2.38%~5.32%;甲维盐在黄瓜和土壤中平均回收率为85.88%~98.21%,RSD 2.44%~5.87%;本法前处理便捷,分析结果准确度高。

The HPLC-FLD method was described for the determination of Abamectin and Emamectin Benzoate in cucumber and soil. The cucumber and soil samples were ultrasonic-extracted with acetonitrile, and derivatization by NMIM and TFAA under the dark condition. The derivative was detected by HPLC-FLD. The linear relationship of Abamectin was found better in the concentration range from 0.01 to 2.0 mg/m L with the linear correlation of 0.9998. The linear relationship of Emamectin Benzoate was found better in the concentration range from 0.01 to 2.0 mg/m L with the linear correlation of 0.9993. The lowest limit of quantification was 0.01 mg/kg. The average recovery of Abamectin was 87.12 %~101.13 %, RSD was 2.38 %~5.32 %. At the addition level of 0.01~1 mg/kg. The average recovery of Emamectin Benzoate was 85.88 %~98.21 %, RSD was 2.44 %~5.87 % at the addition level of 0.01~1 mg/kg.