电针对中枢Stat5敲除所致肥胖小鼠瘦素水平和下丘脑Kctd15、Sh2b1 mRNA的影响

Effects of Electro-acupuncture on Serum Leptin Content and Expression of Kctd15 and Sh2b1 mRNA in Hypothalamus in Stat5-knockout Induced Obese Mice

目的观察电针对中枢Stat5条件性敲除Stat5Nestin-Cre（Stat5NKO）肥胖小鼠下丘脑Kctd15、Sh2b1基因表达的影响。方法 20只肥胖的Stat5NKO小鼠随机分为肥胖组和电针组,每组10只;10只Stat5fl/fl小鼠作为正常组。其中电针组选取单侧后三里、内庭穴进行针刺治疗,每日1次,双侧交替进行,每次30min,韩氏电针仪频率2/15Hz,疏密波,6d为1个疗程,共治疗4个疗程。运用ELISA法检测血清中瘦素（Leptin）含量,qPCR技术检测下丘脑中肥胖相关基因Kctd15、Sh2b1mRNA的表达。结果与正常组小鼠比较,肥胖组小鼠体质量明显增加（P〈0.01）伴随血清Leptin含量升高（P〈0.05）,下丘脑Leptin水平下降（P〈0.05）,同时下丘脑Kctd15、Sh2b1mRNA表达下调（P〈0.05～0.01）;与肥胖组小鼠比较,电针组小鼠的体质量明显下降（P〈0.01）,血清Leptin含量降低（P〈0.01）,且下丘脑Leptin水平回升（P〈0.01）,Kctd15、Sh2b1mRNA表达水平上调（P〈0.01）。结论电针可能通过调节Leptin水平并促进Kctd15、Sh2b1表达,实现对Stat5NKO小鼠的减肥效应。

OBJECTIVE To observe the effect of electro-acupuncture on central conditional knockout Stat5Nestin-Cre obese mice（Stat5NKO）and detect how the obesity related genes,Kctd15 and Sh2b1,are involved.METHODS 20 obese Stat5 NKO mice were randomly divided into two groups：obese model group（n=10）and electro-acupuncture（EA）group（n=10）;10 Stat5fl/fl mice were randomly selected and fed with ordinary forage as normal control group.EA group was treated with electro-acupuncture at Housanli and Neiting points for 30 min,with bilateral alternates,by Han＇electro-acupuncture apparatus at 2/15 Hz.6dwas a period of treatment,with a total of 4courses of treatment.Serum Leptin content was measured by ELISA,and the levels of Sh2b1 and Kctd15 mRNA in hypothalamus were detected by qPCR.RESULTS Compared with Stat5fl/fl mice,Stat5 NKO mice gained body weight significantly（P〈0.01）,accompanied with increased serum Leptin level（P〈0.05）,decreased hypothalamic Leptin level（P〈0.05）,as well as Kctd15 and Sh2b1 mRNA expressions（P〈0.05～0.01）;Compared with Stat5 NKO mice,EA group decreased body weight（P〈0.01）and serum Leptin mRNA level（P〈0.01）.The hypothalamic Leptin level was elevated（P〈0.01）,and the expressions of Kctd15 and Sh2b1 mRNA were returned to normal level（P〈0.01）.CONCLUSION The weight-loss effect of electro-acupuncture on Stat5 NKO obese mice may be related to the regulation of the expression of Leptin,Kctd15 and Sh2b1.