丹皮酚对动脉粥样硬化大鼠炎症因子的影响

Effects of Paeonol on Inflammatory Factors in Rats with Atherosclerosis

目的:探讨丹皮酚对动脉粥样硬化(AS)大鼠血清炎性因子的影响。方法:采用高脂饲料喂养和维生素D3腹腔注射的方法建立大鼠AS模型,分为正常组、模型组、丹皮酚高剂量组(20 mg·kg-1)、丹皮酚低剂量组(10 mg·kg-1),每组8只。灌胃给药4周后,各组取血清,ELISA法测定肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)水平;取主动脉切片,光镜下作病变分级评分,免疫组化法检测单核细胞趋化蛋白-1(MCP-1)和细胞间黏附分子-1(ICAM-1)的表达。结果:丹皮酚高剂量组能改善AS大鼠的主动脉病变,与模型组比较有统计学意义(P<0.05);与模型组比较,丹皮酚可明显降低血清TNF-α和IL-6水平(P<0.01);丹皮酚可显著下调主动脉MCP-1和ICAM-1的表达,与模型组比较差异有统计学意义(P<0.05或P<0.01)。结论:丹皮酚治疗大鼠AS的作用机制与降低炎性因子的表达、抑制炎症反应有关。

Objective： To investigate the effects of paeonol on inflammatory factors in rats with atherosclerosis（ AS）.Methods： Rats model of AS was established by administration of high- fat diet and vitamin D3（ i. p.）. Rats were divided into 4 groups（ n = 8） at random,namely normal,model and paeonol groups（ at doses of 20 mg · kg- 1and 10 mg ·kg- 1）. After 4 weeks of oral treatment,the sera of all rats were collected to determine the levels of tumor necrosis factor alpha（ TNF- α） and interleukin- 6（ IL- 6） by ELISA. The aortic pathologic changes of AS rats were observed under microscope and graded and aortic monocyte chemotactic protein 1（ MCP- 1） and intercellular adhesion molecule 1（ ICAM- 1） activities were detected by immunohistochemistry. Results： Administration of paeonol improved the severity of aortic pathology in AS rats. The pathologic grade in paeonol group（ 20 mg kg- 1） showed a significant difference compared with that of model group（ P〈 0. 05）. Paeonol markedly decreased the TNF- α and IL- 6 levels in serum of AS rats,which showed significant differences compared to those of model group（ P〈 0. 01）. Meanwhile,paeonol significantly inhibited the aortic MCP- 1 and ICAM- 1 activities in AS rats（ P〈0. 05 or P〈 0. 01）. Conclusion： Paeonol shows certain therapeutic effect on AS rats and the mechanisms may be involved in inhibiting the expressions of some inflammatory factors such as TNF- α,IL- 6,MCP- 1 and ICAM- 1.