氧自由基、HMGB1在脓毒症大鼠心肌组织中的动态表达及丹红注射液的干预影响

Expressions of Oxygen-free Radicals and HMGB1 in Heart Tissue of Rats with Sepsis and Effect of Danhong Injection

目的:观察丹红注射液对脓毒症大鼠心肌损伤的保护作用并探讨其可能机制。方法:雄性Wistar大鼠分为6组:正常组、假手术组、模型6 h组、模型24 h组、丹红6 h组和丹红24 h组,每组各8只。盲肠结扎并穿孔(CLP)法建立脓毒症大鼠动物模型,观察各组心肌组织超微结构改变,心肌组织丙二醛(MDA)、超氧化物岐化酶(SOD)和高迁移率族蛋白1(HMGB1)含量的变化。结果:CLP术后,模型组大鼠心肌组织MDA含量、HMGB1蛋白合成进行性升高、SOD活力则持续降低,与假手术组比较有差异(P<0.05),丹红组较模型组同时间点MDA、HMGB1及SOD均有改善,改善有统计学差异(P<0.05);超微结构示模型组大鼠心肌细胞线粒体肿胀,部分肌纤维稀疏,至24 h时肿胀加重,部分空泡变性;丹红组心肌细胞损伤轻于模型组同时间点。结论:丹红注射液对脓毒症大鼠心肌损伤有一定程度的保护作用,其机制可能与清除氧自由基,降低HMGB1含量有关。

Objective： To investigate whether Danhong Injection has a protective effect on myocardial injury in rats with cecal ligation and puncture（ CLP）- induced sepsis and observe the mechanism of the protective effect. Methods： Wistar rats were randomly divided into six groups： normal group,sham- operated group,6 hour model group,24 hour model group,6 hour Danhong Injection group and 24 hour Danhong Injection group. There were 8 rats in each group. To make sepsis rat model by cecal ligation and puncture. The differences of ultrastructure of myocardium cells were observed and compared in each group. At the same time,the following items in heart tissue were observed： the Malondialdehyde（ MDA） contents,the Superoxidedismutase（ SOD） contents and the protein synthesis of High mobility group box- 1 protein（ HMGB1）. Result： The MDA contents and the protein synthesis of HMGB1 in model group were continualy increased after CLP while the SOD contents continuously decreased and the changes were different from those in sham- operated group（ P 〈0. 05）. Compared with model group,Danhong Injection group had lower MDA contents,lower HMGB1 contents and higher SOD contents at the same time（ P 〈0. 05）. The mitochondrion of heart cells in 6 hour model group got swelling and the muscle fiber of heart cells got loose. 24 hours after CLP,most heart cells became autopepsia. Above changes were not more striking in Danhong groups than those in model groups at the same time. Conclusion： Our results suggest that Danhong Injection can protect myocardial injury in sepsis and this effect is related to scavenging oxygen- free radicals and reducing the protein synthesis of HMGB1.