皱瘤海鞘醇提物抗肿瘤生物活性的研究

Studies on anti-tumor activity of Styela plicata ethanol extracts

目的研究皱瘤海鞘醇提物(SPE)的抗肿瘤活性。方法采用四甲基偶氮唑盐(MTT)法检测SPE 0.5、1.0、2.0、4.0和6.0 mg/ml对HuH-7、SW579、SGC7901、MCF-7肿瘤细胞和NIH3T3非肿瘤细胞增殖的影响。分别采用QRT-PCR和蛋白印迹检测SPE(2.0 mg/ml)对不同肿瘤细胞和NIH3T3非肿瘤细胞内Bax、Bcl-2、Caspase-3、Caspase-9的mRNA水平和蛋白水平。结果与空白组相比,1.0、2.0、4.0和6.0 mg/ml SPE组对受试肿瘤细胞的增殖均有不同程度的抑制作用(P<0.05)。2.0 mg/ml SPE可引起受试肿瘤细胞内Bax的m RNA和蛋白水平升高(P<0.05),Bcl-2降低(P<0.05),但Caspase-3和Caspase-9水平无明显变化。此外,SPE对NIH3T3非肿瘤细胞增殖及细胞内Bax、Bcl-2、Caspase-3、Caspase-9的mRNA水平以及蛋白水平影响均无统计学意义。结论 SPE可能通过线粒体膜上的Bcl-2家族触发线粒体凋亡通路,抑制肿瘤细胞增殖。

Objective To investigate the anti-tumor activity of Styela plicata ethanol extracts （SPE）. Methods HUH-7, SW579, SGC7901, MCF-7 and NIH3T3 cells were exposed to SPE 0.5, 1.0, 2.0, 4.0 and 6.0 mg/ml respectively. The proliferation of these tumor cells were detected by 3- （4,5-dimethylthiazol-2-yl） -2,5 -diphenylterazolium bromide （MTT） assay. QRT-PCR and Western blotting were used to measure the mRNA and protein expression of Bax, Bcl-2, Caspase-3 and Caspase-9 in these tumor cells and NIH3T3 cells. Results Compared with non-SPE group, the SPE had obvious impact on these tumor cells proliferation in the concentration of 1.0, 2.0, 4.0 and 6.0 mg/ml （P〈0.05）. The mRNA and protein expression of Bax in these tumor cells group were significantly increased compared with non-SPE group, while Bcl-2 were all decreased. SPE had little influence on the proliferation and expression of NIH3T3 cells. Conclusion The SPE is involved in the mitochondrial apoptosis pathway of tumor cells to play antitumor activity.