大肠癌细胞FoxQ1与EGFR基因表达的相关性

Relationship Between FoxQ1 and Epidermal Growth Factor Receptor Gene Expression in Colorectal Cancer

目的探讨大肠癌细胞中FoxQ1与EGFR基因间的相关性,为研究大肠癌中FoxQ1基因在EGFR通路中的作用机制奠定基础。方法应用荧光定量PCR以293-T细胞中FoxQ1及EGFR基因相对表达量为1作为参照,检测大肠癌细胞系DLD1、HT29、LOVO、HCT116中FoxQ1及EGFR基因mRNA相对表达量;荧光定量检测经shRNA-FoxQ1慢病毒干扰后的DLD1细胞(命名为DLD1-shRNAFoxQ1)中EGFR的相对表达量改变;DLD1-shRNA-FoxQ1经EGFR酪氨酸激酶抑制剂Erlotinib HCl和siRNA-EGFR处理后,荧光定量PCR分别检测FoxQ1和EGFR基因mRNA相对表达量。结果(1)FoxQ1在DLD1、HT29、LOVO、HCT116细胞系中的相对表达量分别为83.09、59.58、0.06、0.03,EGFR的相对表达量分别为4.95、3.67、2.08、1.36;(2)经shRNA-FoxQ1干扰的DLD1细胞EGFR表达量随FoxQ1表达量的降低而增高;(3)细胞DLD1-shRNA-FoxQ1、DLD1-shRNA-Control分别经siRNA-EGFR处理抑制EGFR的表达后,FoxQ1表达量随EGFR表达量的降低而增高,经Erlotinib HC1阻断EGFR酪氨酸激酶后,FoxQ1表达量增高。结论大肠癌细胞系中FoxQ1与EGFR基因的表达趋势基本一致;同时两者间可能相互存在负反馈调节机制,从而维持大肠癌细胞中FoxQ1与EGFR高表达的状态。

Objective To discuss the relationship between Fox Q1 and epidermal growth factor receptor（EGFR） gene expression in colorectal cancer,to provide basis for researching the mechanism of Fox Q1 gene in EGFR pathway.Methods The m RNA relative expression of Fox Q1 and EGFR genes in colorectal cancer cell lines 293-T were taken as reference.We detected the relative m RNA expression of Fox Q1 and EGFR genes in colorectal cancer cell lines DLD1,HT29,LOVO and HCT116.Real-time PCR was used to detect the m RNA relative expression of EGFR in DLD1 cells which was interfered by lentivirus（named DLD1-sh RNA-Fox Q1）;After DLD1-sh RNA-Fox Q1 was processed by EGFR tyrosine kinase inhibitor Erlotinib HCl and si RNAEGFR,we detected the m RNA relative expression of Fox Q1 and EGFR genes by Real-time PCR.Results（1） Relative expression of Fox Q1 and EGFR in DLD1,HT29,LOVO,HCT116 were 83.09,59.58,0.06,0.03 and 4.95,3.67,2.08,1.36,respectively;（2） EGFR expression in DLD1 cells interfered by sh RNA-Fox Q1 was increased with the decrease of Fox Q1 expression;（3） After the expression of EGFR in DLD1-sh RNA-Fox Q1 and DLD1-sh RNA-Control cells were inhibited by si RNA-EGFR,Fox Q1 expression was increased with the decrease of EGFR expression.When EGFR tyrosine kinase was blocked by Erlotinib HCl,Fox Q1 expression was increased.Conclusion Fox Q1 and EGFR gene expression trend are consistent in colorectal cancer cell lines.When Fox Q1 expression is inhibited in colorectal cancer,EGFR expression would be increased.While the expression or protein kinase activity of EGFR is inhibited,Fox Q1 expression would be increased.It is suggested that there may be a negative feedback regulation mechanism between Fox Q1 and EGFR gene expression,which could maintain the high expression of Fox Q1 and EGFR in colorectal cancer cells.