缺氧微环境下TRPC3促进U87MG胶质瘤细胞的增殖

Transient receptor potential canonical channels 3 promotes the proliferation of U87MG glioma cells under hypoxia

目的:探讨缺氧微环境下瞬时感应性C通道3(transient receptor potential canonical channels 3,TRPC3)对U87MG胶质瘤细胞增殖的影响。方法:缺氧建立缺氧模型,以阻断剂抑制通道开放,以RNA干扰技术下调TRPC3的表达;CCK-8检测胶质瘤细胞增殖,流式细胞术检测细胞周期。结果:缺氧条件下TRPC1通道阻断剂及表达下调均可显著抑制U87MG细胞增殖,并引起G1期周期的阻滞。结论:缺氧微环境下TRPC3促进U87MG胶质瘤细胞的增殖。

Objective： To investigate the role of transient receptor potential canonical channels 3（ TRPC3） in the proliferation of glioma cells under hypoxia. Methods： The hypoxic model was established by treating the cells with 3%oxygen or 150μmol / L cobalt chloride. The TRPC3 was knocked down by special small interfering RNA（ siRNA）,and the expression of TRPC3 was evaluated by Real-time PCR and Western Blot. The cells proliferation was detected by CCK-8. The cells cycle was detected by flow cytometry. Results： The proliferation of glioma cells was significantly suppressed by TRPC channel blockers or knock down TRPC3 under hypoxia,and down-regulation of TRPC3 caused cell cycle arrest in G1 phases. Conclusion： TRPC3 promotes the proliferation of glioma cells under hypoxia.