口咽鳞癌中HPV感染与血管生成的关系

The correlation of HPV infection with angiogenesis in oropharyngeal squamous cell carcinomas

目的:研究口咽鳞癌(oropharyngeal squamous cell carcinomas,OSCC)中人乳头瘤病毒(human papillomavirus,HPV)感染与血管内皮生长因子(vascular endothelial growth factor,VEGF)、CD31标记的微血管密度(microvessel density,MVD)表达的关系,探讨它们在口咽鳞癌发生中的相互关系。方法:采用多重实时荧光定量聚合酶链反应(multiplex real-time polymerase chain reaction,MT-PCR)检测60例口咽鳞癌HPV DNA及型别,通过HPV DNA分析进行HPV感染状况的测定;同时采用半定量免疫组化检测HPV(+)组和HPV(-)组中VEGF、CD31(MVD标志)蛋白表达情况。结果:口咽鳞癌中HPV感染率36.7%(22/60),HPV-16型占所有感染者的比例86.4%(19/22),明显高于其他型别;VEGF高度表达者出现MVD>15个/高倍视野的几率较VEGF低度表达者高(P=0.001);MVD>15个/高倍视野的肿瘤为低分化的可能性较高(P=0.004);VEGF和MVD表达与患者年龄、性别、TNM分期、HPV感染状况之间无相关性。结论:HPV感染与口咽鳞癌的发生存在相关性。口咽鳞癌中HPV感染状况和VEGF之间无相关性。

Objective： To study the relationship between human papillomavirus（ HPV） infection and the expression of vascular endothelial growth factor（ VEGF）,microvessel density（ MVD） in 60 oropharyngeal squamous cell carcinomas（ OSCC）. To investigate their correlation in the oncogenesis of oropharyngeal cancer. Methods： HPV status was determined by HPV multiplex real-time polymerase chain reaction（ MT-PCR）. Expression of VEGF and CD31（ as marker of MVD） in HPV（ ＋） and HPV（-） group was assessed by semiquantitative immunohistochemistry. Results： The infection rate of HPV in OSCC was 36. 7%（ 22 /60）. The infection rate of HPV-16 was 86. 4%（ 19 /22）in all positive specimens was significantly higher than other HPV types. VEGF high expressers were significantly more likely to have MVD of 〉15 per HPF than were low expressers（ P = 0. 001）. Tumours with MVD of 〉15 per HPF were more likely to be poorly differentiated（ P = 0. 004）. There was no evidence of associations between VEGF,MVD and patient age,gender,TNM stage,HPV status. Conclusion： HPV infection has significant correlation with the oncogenesis of OSCC. The lack of an association between HPV status and VEGF in this study was noteworthy. Investigations of head and neck tumour cells will be needed to further clarify.