TLR2 mRNA和TLR4 mRNA在蕈样肉芽肿皮损中的表达

Expression of TLR2 mRNA and TLR4 mRNA in mycosis fungoides lesions

目的:探讨Toll样受体2(TLR2)mRNA和Toll样受体4(TLR4)mRNA在蕈样肉芽肿皮损中的表达水平。方法:采用原位杂交技术检测蕈样肉芽肿皮损和正常皮肤中TLR2 mRNA和TLR4 mRNA的表达。结果:蕈样肉芽肿皮损中TLR2 mRNA和TLR4 mRNA主要表达于表皮基底上层,正常皮肤则表达于表皮的基底层。统计学分析表明TLR2 mRNA和TLR4 mRNA在正常皮肤和蕈样肉芽肿皮损处基底层和基底上层的表达均有显著性差异(P<0.05),且TLR2 mRNA和TLR4 mRNA在蕈样肉芽肿皮损中的表达均高于在正常皮肤中的表达(P<0.05)。结论:TLR2 mRNA和TLR4 mRNA可能在蕈样肉芽肿的发生与发展过程中发挥重要作用。

Objective： To detect the expression of TLR2 mRNA and TLR4 mRNA in mycosis fungoides skin.Methods： By using in situ hybridization,TLR2 mRNA and TLR4 mRNA expression levels were demonstrated in mycosis fungoides lesions and normal tissues. Results： TLR2 mRNA and TLR4 mRNA was highly expressed in the suprabasal layer of mycosis fungoides lesional,while in normal epidermis,TLR2 mRNA and TLR4 mRNA was expressed in the basal layer of epidermis. There was a significant difference between mycosis fungoides lesional and normal epiderm（ P〈0. 05）,and TLR2 mRNA and TLR4 mRNA expression were higher in mycosis fungoides lesional epidermis compared with those of the normal epidermis. Conclusion： TLR2 and TLR4 may play a crucial role in the occurrence and development of mycosis fungoides.