子宫内膜癌患者外周血调节性T细胞的检测及临床意义

目的分析子宫内膜癌患者外周血CD4＋CD25＋CD127＋调节性T细胞（Treg）水平及其在肿瘤免疫耐受中的作用。方法选择2012年8eq至2014年8月子宫内膜癌患者82例为观察组，健康志愿者30例为对照组，采用流式细胞仪检测外周血中CD14＋CD25＋CD127 Treg占CD4＋T细胞的比例，分析其与肿瘤分期、分化程度和年龄的关系。结果子宫内膜癌患者CD4＋CD25＋CD127 Treg占CD4＋T细胞比例为（4．89±1．42）％，显著高于健康对照组（3．34±0．84）％。不同分期、不同分化程度及不同年龄子宫内膜癌患者的CD4＋CD25＋CD127＋Treg占CD4＋T细胞比例差异无统计学意义。结论子宫内膜癌患者外周血CD4＋CD25＋CD127 Treg占CD4＋T细胞比例明显升高，可能参与子宫内膜癌免疫耐受。

Objective to investigate the frequency ofCD4＋ CD25＋ CD12T regulatory T cells in CD4＋ T cells in peripheral blood of the patients with endometrial cancer. Methods 82 patients with emdometrial cancer admitted in Zhejiang Tumor Hospital from August 2012 to August 2014 and 30 healthy women （ controls ） were included in this study. The frequency of CD4＋ CD25＋ CD127 regulatory T cells in CD4＋ T cells in peripheral blood was detected by flow cytometry and its relation with tumor stage and differentiation degree was analyzed. Results The frequency of CD4＋ CD25＋ CD12T regulatory T cells in CD4＋ T cells in peripheral blood of 82 patients with endometrial cancer was （ 4.89 ± 1.42 ） %, significantly higher than that in control group. But no significant difference was found with that in tumor stage and differentiation degree. Conclusion Compared with that in healthy women, the frequency of CD4＋ CD25＋CD127＋ Treg in the peripheral blood of endometrial cancer patients showed a significant increase, which may be responsible for immune tolerance in endometrial cancer.