摘要
目的观察神经生长因子(NGF)对分离的新生大鼠脑细胞内游离钙浓度([Ca2 + ]i)的影响。方法以Fura -2/AM为细胞内钙离子的荧光指示剂 ,测定谷氨酸(Glu)及过氧化氢(H2O2)诱导的新生大鼠脑细胞内[Ca2 + ]i,观察NGF对此影响。结果新生大鼠脑细胞内静息[Ca2 + ]i208±22nmol/L,NGF对神经细胞静息[Ca2 +]i无明显影响 ,NGF1～100μg/L能剂量依赖地抑制Glu和H2O2 引起的[Ca2 + ] i升高 ,其IC50 分别为42 5和27 3μg/L。结论NGF通过降低[Ca2 +
Aim Effects of nerve growth factor (NGF) on intracellular free Ca2+ concentration (i) in isolated brain cells of neonatal rat were observed. Methods i was measured with Ca2+ sensitive fluorescent indicator, Fura-2/AM, in isolated brain cells of neonatal rat, and the effects of nerve growth factor (NGF) on the changes in i induced by glutamate (Glu) and hydrogen perioxide (H2O2) were studied. Results The resting i was 208 ±22 nmol/L when the brain cells were incubated in Hanks' solution containing Ca2+ 1.3nmol/L. NGF did not alter the resting i in brain cells. NGF 1～100μg/L dose-dependently attenuated glutamate (0.5 mmol/L) and hydrogen peroxide (0.05 mmol/L) induced elevation of i by 10%, 18%, 32%, 44%, 62% and 14%, 30%, 39%, 49%, and 65%, respectively, with an IC50 of 42.5 and 27.3μg/L (95% confidence limits:28.1～71.5 and 18.1～46.5 μg/L). Conclusion These suggest that NGF protect cortical neurons against glutamate toxicity and oxidative insults via reducing the elevation of i or stabilizing i level.
