碱性β-葡萄糖苷酶产生菌株的筛选、鉴定及部分酶学性质研究

Screening and identification of alkaline β-glucosidase producing-strain and partial enzyme characterization

为开发新型碱性微生物来源的β-葡萄糖苷酶资源,本文采用七叶苷平板显色筛选法在碱性条件下从多个土壤样本和印染洗涤废水中筛选得到一株β-葡萄糖苷酶产生菌株,并对该菌株进行了分子生物学鉴定、发酵特性及部分酶学性质研究。结果表明:筛选菌株为Bacillus sp.,其最适生长和产酶温度为35~37℃,最适生长和产酶p H分别为9.5和9.0;该菌株所产β-葡萄糖苷酶最适作用温度和p H分别为50℃和9.5;在50℃以下和p H9.0~10.0内比较稳定;Cu^(2+)、Fe^(2+)、Ca^(2+)和Mn^(2+)对该酶表现出一定抑制作用,而Mg^(2+)、Zn^(2+)和Co^(2+)对该酶催化活性没有明显影响。研究结果对产碱性β-葡萄糖苷酶的微生物资源获取和信息整理具有一定的借鉴意义。

One β-glucosidase producing strain was selected from various soil samples and dyeing wastewater by using esculin agar plate screening method with the aim to study and develop alkaline microbial origin β-glucosidase resources,and the microbiology identification,fermentation properties and enzyme characterization of the isolated strain were further studied. The isolated strain was identified as Bacillus sp.,and the optimum growth and enzyme production temperatures was between 35 37 ℃,and the optimum growth and enzyme production p H were at 9.5 and 9.0,respectively. The optimum temperature and p H of the produced enzyme was 50 ℃ and 9.5,respectively,and the enzyme was stable at temperatures below 50 ℃ and in a p H range of9.010.0. Mental ions,such as Cu2＋,Fe2＋,Ca2＋ and Mn2＋ showed some inhibition to the enzyme,Mg2＋,Zn2＋ and Co2＋have no significantly effect on the catalytic activity of the enzyme. The results obtained in the study were meaningful for microbial resources and information consolidation of alkaline β-glucosidase producing strain.