高产信号分子AI-2乳酸菌的筛选及鉴定

Screening and identification of AI-2 high-producing lactic acid bacteria

通过V.harveyi BB170生物学方法检测分离自内蒙古锡盟地区的8株乳酸菌产生群体感应信号分子AI-2的情况,筛选出高产信号分子AI-2的菌株进行分子生物学鉴定,并探究信号分子AI-2变化规律。结果表明:8株乳酸菌均能够分泌具有活性的信号分子AI-2,其中,菌株2-1产信号分子AI-2的能力显著优于其他7株乳酸菌(p<0.05);高产AI-2的乳酸菌菌株2-1经鉴定为发酵乳杆菌(Lactobacillus fermentum);随着2-1菌体的生长信号分子AI-2的浓度也随之增大,并且信号分子AI-2浓度在稳定期初期达到最大值。

The V. harveyi bioassay method was carried out to explore that 8 lactic acid bacteria which isolated from koumiss of Ximeng region in Inner Mongolia produced the quorum-sensing signal autoinducer-2（AI-2）and found the production pattern. AI-2 high-producing strain was screened for molecular biological identification and relationship between AI-2 and bacterial density was further studied. The results showed that all strains could produce AI-2,among of which the ability of AI-2 production of strain 2-1 was superior（p〈0.05） and identified as a Lactobacillus fermentum. The concentration of AI-2 increased with bacterial density and the largest amount of AI-2 was observed in the early stationary phase in the culture of strain 2-1.