DEAE-琼脂凝胶微球的制备及在R-PE和C-PC提取分离中的应用

The preparation of DEAE-agar gel microspheres and its application in the separation of R-Phycoerythrin and C-Phycocyanin

为了降低天然大分子功能蛋白的分离纯化成本,以琼脂为原料自制弱阴离子交换层析介质DEAE-琼脂凝胶微球。将DEAE-琼脂凝胶微球应用于坛紫菜R-藻红蛋白（R-PE）和螺旋藻C-藻蓝蛋白（C-PC）的提取分离,最佳洗脱条件为：层析柱规格h=35.0 cm,φ=1.0 cm,V=27.5 m L;流速v=0.33 m L/min;洗脱缓冲液为0.01 mol/L磷酸盐溶液（含有0.05~0.5 mol/L Na Cl溶液）;0.01 mol/L磷酸盐缓冲液（含0.1 mol/L Na Cl）洗脱出纯度为5.0的坛紫菜R-PE;0.01 mol/L磷酸盐缓冲液（含0.2 mol/L Na Cl）洗脱出纯度为4.4的螺旋藻C-PC。自制层析介质成本低廉、耗能少、可大量制备、分离效果好,可用于坛紫菜R-PE、螺旋藻C-PC的产业化制备。

In order to reduce the separation and purification cost of natural macromolecular functional protein,a kind of weak anion exchange chromatography medium DEAE-agar gel microsphere was made up. Applied DEAE-agar gel microsphere to the separation and purification of the porphyra haitanensis R-phycoerythrin（ R- PE） and spirulina C-phycocyanin（ C- PC）, optimal elution conditions were as follows ： chromatography column specifications was h=35.0 cm,φ=1.0 cm,V=27.5 m L,current velocity v=0.33 m L/min,elution buffer solution was 0.01 mol/L phosphate buffer solution（contain 0.05~0.5 mol/L Na Cl）. 0.01 mol/L phosphate buffer solution（contain 0.1 mol/L Na Cl） washed porphyra haitanensis R-PE out,its purity was 5.0. 0.01 mol/L phosphate buffer solution（contain 0.2 mol/L Na Cl） washed spirulina C-PC out,its purity was 4.4. The DEAE-agar gel microspheres could be used in the industrial production of R-PE and C-PC because of their low cost,low energy consumption,scale preparation and excellent separation effect.