植物乳杆菌SCS1对高血糖模型小鼠的降血糖作用

Effect of Lactobacillus plantarum SCS1 on blood glucose levels in hyperglycemia model mice

本文通过给小鼠灌胃植物乳杆菌SCS1(Lactobacillus plantarum SCS1,L.plantarum SCS1)发酵液研究其对高血糖模型小鼠的体重、血糖水平、葡萄糖耐受量、血浆中离子(K^+、Na^+、Cl^-)含量以及肝糖原等指标的影响。通过选用3周龄SPF(specific-pathogen free)级昆明(KM)雄性小鼠,随机分为普通组(NG)、预防组(PG)、模型组(MG)、低剂量治疗组(TGL)、中剂量治疗组(TGM)和高剂量治疗组(TGH)。其中NG小鼠灌胃10 m L/kg体重MRS(De Mann,Rogosa and Sharpe)液体培养基作为空白对照组,PG小鼠第二周开始采用普通饲料和10 m L/kg体重L.plantarum SCS1发酵液灌胃一周,MG小鼠灌胃10 m L/kg灭菌生理盐水为模型对照组,TGL、TGM和TGH分别灌胃10 m L/kg浓度为2.0×10~9、4.0×10~9和6.0×10~9 CFU/m L的L.plantarum SCS1发酵液。对除NG以外的各组小鼠连续注射70 mg/kg体重的链脲佐菌素(Streptozocin,STZ)诱导并辅以高脂高糖饲料喂养一周,建立高血糖小鼠模型。实验结果表明,与MG小鼠血糖水平相比,高浓度(6.0×10~9 CFU/m L)L.plantarum SCS1发酵液对于TGH小鼠的血糖水平有显著降低作用(p<0.05),但预防作用不显著。另外,与MG小鼠相比,L.plantarum SCS1发酵液可以显著提高STZ诱导的TGH小鼠葡萄糖耐受能力(p<0.05)。TGH小鼠血浆中K^+含量较MG小鼠显著升高(p<0.05),而其血浆中Na^+和Cl^-含量显著降低(p<0.05)。再者,与MG小鼠相比,L.plantarum SCS1发酵液对TGH小鼠的肝糖原含量也显著降低(p<0.05)。因此,L.plantarum SCS1发酵液具有降低高血糖模型小鼠血糖水平的作用,同时能够改善由于高血糖引起的如小鼠体重减轻、血浆中K^+的丢失,Na+和Cl^-含量的升高以及肝糖原含量升高等问题。

The effect on body weight and blood glucose levels in hyperglycemia model mice by administrating Lactobacillus plantarum SCS1（L. plantarum SCS1） was researched in the study.Meanwhile,glucose tolerance,content of K~＋,Na＋and Cl^- in plasma and content of glycogen were also studied. Sixty 3-week-old male KM mice in a SPF grade were divided into six groups randomly,including NG,MG,PG,TGL,TGM and TGH. NG mice were fed with MRS medium（ 10 m L / kg body weight）. PG mice were fed with concentration of 6. 0 ×109CFU/m L L. plantarum SCS1 suspensions from the second week to third week. MG mice were fed with sterile physiological saline（10 m L/kg body weight）. TGL,TGM and TGH mice were fed with concentration of2.0×10~9,4.0×10~9and 6.0×10~9CFU/m L L. plantarum SCS1 suspensions（10 m L/kg body weight） respectively from the fourth week to the end of the experiments. The hyperglycaemia model were established with 1-week high diet plus with 70 mg/kg of STZ injection. The results showed that the blood glucose of TGH mice were reduced by high dose（6.0 ×10~9CFU/m L） fermentation broth of L. plantarum SCS1 significantly（p 〈0.05）compared with mice,and the preventive effect was not significant. Otherwise,the glucose tolerance of TGHmice were decreased by fermentation broth of L. plantarum SCS1 significantly（p〈0.05）,content of K~＋ in plsma of TGH mice were increased,content of Na＋and Cl^- in plasma and glycogen were decreased by fermentation broth of L. plantarum SCS1 significantly（ p 〈 0. 05） compared with MG mice. These results suggested that L. plantarum SCS1 fermentation broth showed a hypoglycemic effect on hyperglycaemia model mice. Loss of K~＋,increased Na＋,Cl-in plasam and glycogen caused by hyperglycaemia were improved by L. plantarum SCS1 fermentation broth.