加味二陈汤对ApoE^(-/-)小鼠炎症标志物IL-6和TNF-α的调节作用

The Effects of Modified “Erchen Decoction”on the Expression of Inflammatory Marker IL-6,TNF-α on Apo E^(-/-) Mice

目的研究加味二陈汤对Apo E-/-小鼠血清、主动脉壁及瓣膜的IL-6、TNF-α表达的影响。方法40只Apo E-/-小鼠中10只为空白对照组,30只以高脂饲喂养4周构建动脉粥样硬化(As)小鼠模型,均分成模型组、辛伐他汀组、加味二陈汤组,分别用生理盐水、辛伐他汀及加味二陈汤对小鼠灌胃给药,8周后处死小鼠,Elisa法检测血清中IL-6、TNF-α的含量;免疫组化法检测IL-6、TNF-α在主动脉壁及瓣膜的表达。结果辛伐他汀组血清IL-6和TNF-α分别为392.06±37.70 pg/m L、189.1±50.4 pg/m L,与模型组相比表达下降(P<0.05);加味二陈汤组血清IL-6、TNF-α分别为442.41±172.26 pg/m L、47.2±13.3 pg/m L,与模型组相比表达下降(P<0.05),与辛伐他汀组相比,表达下降(P<0.05);免疫组化结果显示加味二陈汤可降低IL-6、TNF-α在主动脉壁内的表达。结论加味二陈汤可降低血清和主动脉壁及瓣膜的IL-6、TNF-α的表达。

Objective This study was to investigate the effects of Modified Erchen Decoction on the expression of IL-6,TNF-αin the aortic wall and serum of ApoE-/ - mice,in order to confirm the effect on the regulation of inflammatory factors in atherosclerosis. Methods ApoE-/ - mice of the control group （n=10） were fed with normal diet,the others （n=30） were fed with a high-cholesterol diet in 4 weeks to induce atherosclerosis mode,and then were divided into the model group （n=10）,the Modified Erchen Decoction group （n=10） and the simvastatin group （n=10）. The two treatment groups were given Modified Erchen Decoction and simvastatin by gavage respectively. The expression of IL-6 and TNF-αwas measured by ELISA kit after treated with the drugs for 8 weeks continuously. Results IL-6 and TNF-α in serum were 392. 06 ± 37. 70 pg/mL and 189. 1 ± 50. 4 pg/mL in the simvastatin group and were 47. 2 ± 13. 3 pg/mL and 442. 41 ± 172. 26 pg/mL in the Modified Erchen Decoction group respectively. They were decreased by the two drugs,and significant difference was found between each treatment group and the control group. The expression of IL-6 and TNF-α were decreased by the two drugs,which were confirmed by the histopathology. Conclusion Modified Erchen Decoction can inhibit the expression of IL-6 and TNF-αeffectively in the ApoE-/ - mice,which indicates that Chinese formula may reduce atherosclerosis progress.