非小细胞肺癌组织中VEGF和Bax的表达及意义

Expression and significance of VEGF and Bax in non-small cell lung cancer

目的观察非小细胞肺癌（NSCLC）中血管内皮生长因子（VEGF）和Bax的表达,并探讨其临床应用价值。方法收集该院NSCLC患者肺组织石蜡标本80例（NSCLC组）及良性病变病灶旁正常肺组织石蜡标本20例（对照组）,采用Western blot和免疫组织化学染色（SP）法测定NSCLC组织中VEGF和Bax的表达,并探讨二者与NSCLC病理类型、TNM分期及淋巴结转移的相关性。结果 NSCLC组中VEGF阳性表达显著高于对照组,Ⅲ~Ⅳ期VEGF阴性表达高于Ⅰ~Ⅱ期,有淋巴结转移组织中的VEGF阳性表达高于无淋巴结转移组织,差异均有统计学意义（P〈0.05）;VEGF的表达与腺癌、鳞癌无相关性（P〉0.05）。NSCLC组中Bax表达低于对照组,Ⅲ~Ⅳ期Bax阳性表达低于Ⅰ~Ⅱ期,有淋巴结转移组织中Bax阳性表达低于无淋巴结转移组织,差异均有统计学意义（P〈0.05）;Bax的表达与腺癌、鳞癌无相关性（P〉0.05）。NSCLC中VEGF和Bax的表达呈负相关。结论 VEGF的表达在NSCLC中起着促进作用,并与Bax表达呈负相关,二者的表达可为NSCLC的诊断及预后提供判断依据。

Objective To investigate the expression of vascular endothelial growth factor （VEGF） and Bax in non-small cell lung cancer （NSCLC） and clinical value. Methods Paraffin specimens were collected from 80 cases of NSCLC tissues（NSCLC group） and 20 cases of normal lung tissue adjacent to benign lesions（control group）. Western blot and immunohistochemistry were used to dectect the expression of VEGF and Bax. The relationship of the expression of VEGF and Bax with histological classifica- tion,stage,lymph mode metastasis were analyzed. Results The expression of VEGF in NSCLC group was higher than that in con- trol group, the differences were statistically significant （P〈0.05）. The higher expression of VEGF in the group of stage Ⅲ-Ⅳ was obtained than that in the group of stage Ⅰ-Ⅱ （P〈0.05）. The higher expression of VEGF in the group with lymph mode metasta- sis was obtained than that in the group without lymph mode metastasis （P〈0.05）. The expression of VEGF was not found to be related with histological classification （P〈0.05）. The expression of Bax in NSCLC tissue was lower than that in normal tissue, the differences was statistically significant （P〈0.05）. The lower expression of Bax in the group of stage Ⅲ-Ⅳwas obtained than that in the group of stage Ⅰ - Ⅱ （P〈0. 05）. The lower expression of Bax in the group with lymph mode metastasis was obtained than that in the group without lymph mode metastasis （P〈0.05）. The expression of Bax was not found to be related with histological classification （P〈0.05）. The expression of VEGF was negatively correlated with Bax in NSCLC （P〈0.05）. Conclusion The ex- pression of VEGF played a promoting effect in the NSCLC, and was negatively correlated with the expression of Bax protein, and the expression of the two could provide a basis for the diagnosis and prognosis of NSCLC.