龙葵碱联合VEGF抗体对人结肠癌鸡胚移植模型血管生成的影响

Effect of angiogenesis on Solanine and VEGF antibody in chicken embryo transplantation model of human colon cancer cells

目的建立人结肠癌鸡胚移植模型,探讨龙葵碱、VEGF抗体及两者联合对人结肠癌细胞诱导肿瘤血管生成及肿瘤增殖的影响。方法将人结肠癌HT-29细胞鸡胚移植模型分为实验组和对照组,实验组加入龙葵碱、VEGF抗体和龙葵碱+VEGF抗体混合液,对照组加入磷酸盐缓冲液(PBS)液。通过立体显微镜照相、IPP 6.0图像分析软件分析图片;免疫组织化学方法检测CD34抗原和ki-67抗原,观察龙葵碱、VEGF抗体和龙葵碱联合VEGF抗体对肿瘤血管生成及肿瘤增殖的影响。结果肿瘤血管面积、CD34抗原和ki-67抗原表达:龙葵碱+VEGF抗体组明显优于单药VEGF抗体组和龙葵碱组,VEGF抗体组优于龙葵碱组,3组均明显优于对照组(P<0.01)。结论龙葵碱、VEGF抗体及两者联合时均能抑制人结肠癌HT-29细胞系诱导的肿瘤血管生成及肿瘤增殖,为抗肿瘤血管生成提供了一种新途径。

Objective To establish model of the chicken embryo transplantation of human colon cancer cells,and investigate the effect of Solanine,VEGF antibody and Solanine combined with VEGF antibody on human colon cancer cells induce tumor anglo- genesis and tumor proliferation. Methods The model of the chicken embryo transplantation of human colon cancer HT-29 cells were divided into three experimental group and control group. We added to the chick embryo chorioallantoic membrane with Sola- nine.VEGF antibody and Solanine＋ VEGF antibody mixture, PBS was added to the control group. Then we analysed picture through the stereomicroscope and IPP 6.0 image analysis software, using immunohistochemistry envision method to detect of CD34 antigen and ki-67 antigen,and observing effect of Solanine group, VEGF antibody group,Solanine＋ VEGF antibody group and the effect on the tumor angiogenesis and tumor proliferation. Results The tumor angiogenesis,CD34 antigen and ki-67 antigen of Sola- nine＋VEGF antibody group were significantly better than those of VEGF antibody group and Solanine group（P〈0.01） ;VEGF antibody group had statistical significant difference with Solanine group（P〈0.01） ;the effect of other three groups were better than that of the control group（P〈0.01）. Conclusion Solanine.VEGF antibody and Solanine combined with VEGF antibody could in- hibit tumor angiogenesis and tumor proliferation of human colon cancer cell line HT-29 to induce. It provides a new way for anti-an- giogenes.