不同受精方式及精子来源对剩余胚胎发育能力的影响

Effects of fertilization methods and sperm sources on the developmental capacity of surplus embryos

目的探讨不同受精方式及ICSI中不同来源精子对胚胎继续发育能力的影响。方法本研究回顾性分析该中心2013年1月至2013年12月2 697例患者剩余胚胎的囊胚率,按不同受精方式分为体外受精(IVF)胚胎组和显微受精(ICSI)胚胎组,其中ICSI胚胎组又根据精子来源不同再分为精液精子组胚胎和附睾精子组,序贯培养后比较不同受精方式及不同来源精子对剩余胚胎的囊胚形成率及优质囊胚率的影响。结果2 697例患者共培养胚胎8 426枚,其中IVF组7 048枚囊胚形成率53.18%,ICSI组1 378枚囊胚形成率49.27%,IVF组囊胚形成率和优质囊胚率之间差异无统计学意义(P>0.05)。同时与常规IVF组相比补救受精组剩余胚胎的囊胚率差异无统计学意义(P>0.05)。常规ICSI组与PESA组的剩余胚胎囊胚形成率和优质囊胚率之间差异无统计学意义(P>0.05)。结论不同受精方式及ICSI精子来源的囊胚率之间差异不明显。

Objective To analyze the effects of fertilization methods and sperm sources in intra-cytoplasmic sperm injection （ICSI） on the developmental capacity of surplus embryos. Methods A retrospective study was carried out to compare the blasto- cyst rate of the surplus embryos from 2 697 patients. According to the fertilization methods, the embryos were divided into IVF group and ICSt group. According to sperm sources,the ICSI group was divided into ejaculated group and testicular sperm group. The rates of blastocyst formation and good quality blastocysts were compared between different fertilization methods and sperm sources. Results There were 8 426 embryo developed in 2 697 patients. Theblastocyst formation rate of surplus embryos was high- er in the IVF group（n= 1 048,53.180% ） than that in the ICSI group（n=1 378,49.27 %）, but with no statistically significant differ- ent（P〈0.05）. The rates of blastocyst were not statistically significant different between the IVF group and in the rescue ICSI group（P〉0.05）. The rates of blastocyst were not statistically significant different between the ejaculated group and the testicular sperm group（P〉0.05）. Conclusion There were not statistically significant different of the rate of blastocyst between different fer- tilization methods and sperm sources in ICSI.