正交法优选丹参提取工艺研究

Optimization of extraction process of Salviae Miltiorrhizae by Orthogonal experiment

目的优选丹参的提取工艺。方法以丹酚酸B及丹参酮ⅡA的提取率为考察指标,对丹参的提取工艺进行正交试验法,筛选出最佳提取工艺。结果优选出的丹参最佳提取工艺为:8倍量70%乙醇在70℃下加热回流提取90 min。结论优选得到的丹参提取工艺稳定可行。

Objective To establish a method to optimize and determine the extraction technology of Salviae Miltiorrhizae. Methods In this article,an orthogonal test was conducted,with salvianolic acid B and tanshinone IIA as indexes,to detect four factors,including the extraction time,extraction temperature,the dosage of ethanol and solvent to solid ratio,and to optimize the extraction process of Salviae Miltiorrhizae. Results Optimized extraction method was as follows： 8 times the amount of 70% ethanol,reflux extraction90 min at 70℃. Conclusion Optimized extraction technology of Salviae Miltiorrhizae is stable and feasible.