AFPmAb-PLGA-rhDCN纳米粒对HepG2细胞迁移、侵袭的影响

Effect of AFPmAb-PLGA-rhDCN nanoparticles on migration and invasion of HepG2 cells

目的探讨甲胎蛋白单抗修饰的荷载人重组核心蛋白聚糖质粒的聚乳酸-羟基乙酸[alpha-fetoprotein monoclonal antibody-poly(lactic-co-glycolic acid)-recombinant human decorin,AFPm Ab-PLGA-rhDCN]纳米粒对肝癌细胞株HepG2细胞迁移和侵袭能力的影响。方法将HepG2细胞分为未处理组、用含空质粒的PLGA纳米粒处理的空白组及低、中、高浓度AFPmAb-PLGA-rhDCN纳米粒处理的实验组,采用划痕试验和Transwell小室试验检测纳米粒对细胞迁移和侵袭的影响,RT-PCR法检测RhoC基因mRNA转录水平,ELISA和Western blot法检测RhoC蛋白的表达水平。结果低、中、高浓度实验组细胞划痕宽度值及穿透膜的细胞数与未处理组及空白组相比,差异均有统计学意义(P均<0.05);低、中、高浓度实验组细胞RhoC基因mRNA灰度值及蛋白浓度均低于未处理组及空白组,差异均有统计学意义(P均<0.05)。结论 AFPm Ab-PLGA-rhDCN纳米粒对HepG2细胞的迁移和侵袭能力具有抑制作用,该抑制作用与下调RhoC分子的表达有关。

Objective To investigate the effect of alpha-fetoprotein monoclonal antibody-poly（lactic-co-glycolic acid）-recombinant human decorin（AFPm Ab-PLGA-rhDCN）nanoparticles on migration and invasion of HepG2 cells. Methods HepG2 cells were randomly divided into untreated group, blank control group（treated with PLGA containing empty plasmid） as well as low, moderate and high concentration AFPm Ab-PLGA-rhDCN nanoparticle groups. The effect of nanoparticles on the migration and invasion of cells were determined by scratch test and Transwell chamber test respectively, while the transcription level of RhoC mRNA by RT-PCR, and expression level of RhoC protein by ELISA and Western blot. Results The width of scratches and counts of transmembrane cells in low, moderate and high concentration AFPm Ab-PLGA-rhDCN nanoparticle groups showed significant differences with, while the gray values of Rho C m RNA and concentrations of Rho C protein were significantly lower than those in untreated and blank control groups（each P〈0.05）.Conclusion The AFPm Ab-PLGA-rh DCN nanoparticles inhibited the migration and invasion of HepG2 cells significantly,which was related to the down-regulation of expression of Rho C molecules.