子痫前期胎盘组织中差异蛋白质表达的研究

Study on differentially expressed proteins in placental tissue of patients with preeclampsia

目的筛查子痫前期胎盘组织中差异表达的蛋白,寻找与子痫前期发生发展相关的蛋白。方法通过蛋白质芯片技术(PPA),针对64个磷酸化或非磷酸化抗体,检测2011年7月-2012年6月就诊的101例子痫前期患者(子痫前期组)及65例健康孕妇(对照组)的胎盘蛋白质表达水平。结果子痫前期组和对照组中有36个蛋白差异表达,19个表达上调,17个表达下调。多因素回归分析发现,Endoglin、PAI-1和Flt-1与子痫前期的不良结局发生相关;Endoglin与早发型子痫前期密切相关;PAI-1与子痫前期胎儿生长受限相关。结论本研究发现胎盘中与早发型子痫前期、不良结局、胎儿生长受限的相关蛋白,这些蛋白可能参与子痫前期的发生发展,是子痫前期预测及病情严重程度分级的潜在指标。

Objective To screen differentially expressed proteins in placental tissue of patients with preeclampsia,find the related proteins of occurrence and development of preeclampsia. Methods Protein pathway array（ PPA） was used to detect the expressions of placental proteins in 101 cases with preeclampsia（ preeclampsia group） and 65 normal pregnant women（ control group） from the hospital from July2011 to June 2012 targeting to 64 phosphorylation or non-phosphorylation antibodies. Results There were 36 differentially expressed proteins in preeclampsia group and control group,19 proteins were upregulated and 17 proteins were downregulated. Multivariate regression analysis showed that Endoglin,PAI-1,and Flt-1 were correlated with adverse outcome of preeclampsia,Endoglin was closely associated with early-onset preeclampsia,PAI-1 was associated with fetal growth restriction among preeclampsia cases. Conclusion This study indentified the associated placental proteins of early onset preeclampsia,adverse outcome,and fetal growth restriction,those proteins may be involved in the occurrence and development of preeclampsia,which can be potentially used for preeclampsia prediction,classification,and risk stratification of preeclampsia.