摘要
目的探讨Caveolin-1对宫颈癌细胞增殖和迁移能力的影响及其机制。方法体外培养宫颈癌细胞株Hela,将细胞株分为实验组、阴性对照组和正常对照组,实验组为运用质粒转染技术建立过表达的稳定细胞株Hela/Caveolin-1,空载体细胞株Hela/pc DNA3.1作为阴性对照组,正常对照组为未转染的常规培养细胞。采用RT-PCR方法检测3组细胞中Caveolin-1的表达水平,MTT方法检测宫颈癌细胞增殖能力,Transwell细胞侵袭实验测定转染Caveolin-1后宫颈癌细胞的侵袭和迁移能力。结果 RT-PCR方法结果说明,Hela/Caveolin-1实验组中Caveolin-1的表达(5.82±0.19)明显高于阴性对照组(1.19±0.01)和正常对照组(1.32±0.06),且实验组Hela细胞的增殖和迁移能力显著减弱。结论 Caveolin-1与宫颈癌细胞的增殖和迁移能力有关。
Objective To explore the effect of Caveolin-1 on proliferation and migration of cervical cancer cells and the mechanism. Methods Cervical cancer HeLa cells were cultured in vitro, then the cells were divided into experimental group, negative control group, and normal control group; experimental group included overexpression HeLa/Caveolin-1 cells established by plasmid transfection technique, negative control group included empty vector HeLa./pcDNA3.1 cells, normal control group included conventional culture ceils without trans- fection. RT-PCR was used to detect the expression levels of Caveolin-1 in the three groups, MTT assay was used to detect proliferation abil- ity of cervical cancer cells, Transwell assay was used to detect invasion and migration of cervical cancer cells transfeeted by Caveolin- 1. Re- stilts The expression level of Caveolin-1 in experimental group was （5. 82±0. 19）, which was significantly higher than those in negative control group （1.19±0.01） and normal control group （1.32±0. 06）, proliferation and migration of cervical cancer cells in experimental group weakened significantly. Conclusion Caveolin-1 is related to proliferation and migration of cervical cancer cells.
出处
《中国妇幼保健》
CAS
2016年第3期608-610,共3页
Maternal and Child Health Care of China
基金
承德市科学研究与发展计划项目(20122138)
承德青年科研基金资助项目(201310)
