尿巨细胞病毒DNA与血清巨细胞病毒抗体检测在小儿巨细胞病毒感染中的应用

Application of urine HCMV- DNA and serum HCMV antibodies detection in children with HCMV infection

目的探讨尿巨细胞病毒DNA与血清巨细胞病毒抗体检测在小儿巨细胞病毒(HCMV)感染中的临床应用价值。方法收集2013年1月-2014年6月在该院就诊的疑似HCMV感染的患儿339例为研究对象。用荧光定量聚合酶链反应(FQPCR)方法检测疑似HCMV感染患儿尿HCMV-DNA,用酶联免疫吸附试验(ELISA)方法检测患儿血清HCMV-Ig G和HCMV-Ig M,比较两种方法的阳性率。结果尿HCMV-DNA和血清HCMV-Ig M阳性率检出率分别为41.00%和45.72%,两者差异无统计学意义,两种方法联合检测的阳性检出率为56.64%,高于单一方法;HCMV-Ig M(+)HCMV-Ig G(-)组、HCMV-Ig M(+)HCMV-Ig G(+)组和HCMV-Ig M(-)HCMV-Ig G(+)组的尿HCMV-DNA阳性率检出率分别为72.50%、63.48%和21.14%,差异有统计学意义。结论联合运用两种检测方法可提高疑似HCMV感染患儿早期诊断的阳性检出率,尿HCMV-DNA对确诊患儿的疗效监测有更好的临床应用价值。

Objective To investigate clinical application value of urine human eytomegalovirus （HCMV） -DNA and serum HCMV antibodies detection in children with HCMV infection. Methods From January 2013 to June 2014, 339 children with suspected HCMV in- fection were selected as research object. Urine HCMV-DNA was determined using reahime PCR, serum HCMV-IgG and HCMV-lgM were determined using ELISA, and then the positive rates of two methods were compared. Results The positive detection rates of urine HCMV- DNA and serum HCMV-IgM were respectively 41.00% and 45.72% , and the difference was not significant. The positive rate of combined detection of the two methods was 56. 64%, which was higher than those of single method. The positive rate of urine HCMV-DNA in HCMV -IgM （＋） and HCMV-IgG （-） group, HCMV-IgM （＋） and HCMV-IgG （＋） group, HCMV-IgM （-） and HCMV-IgG （＋） group were 72. 50%, 63.48%, and 21.14% respectively, the difference had statistical significance. Conclusion The combined application of the two methods can increase the positive detection rate in early diagnosis of children with suspected HCMV infection, and urine HCMV-DNA had better clinical application value for curative effect monitoring among diagnosed children.