柚皮苷对大鼠蛛网膜下腔出血后神经炎症的影响

Effects of Naringin on Cerebral Vasospasm and Apoptosis After Subarachnoid Hemorrhage in Rats

目的研究柚皮苷对蛛网膜下腔出血(subarachnoid hemorrhage,SAH)引起的脑组织神经炎症的影响。方法 54只SD大鼠随机分为假手术组、模型组和柚皮苷组(80 mg·kg^(-1))。各组大鼠过量麻醉致死,断头取脑后干湿重法测水肿;取冻存于液氮的患侧大脑皮质,称重后用裂解液匀浆处理;离心提蛋白,Western blot定量检测炎症相关因子NF-κB;各组大鼠断头取脑后置于4%中性甲醛固定,常规石蜡包埋和切片,免疫荧光染色半定量检测小胶质细胞标记钙离子结合调节因子(ionized calcium-binding adapter molecule 1,Iba-1)和星形细胞标记胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)。结果与模型组比较,柚皮苷能明显减少SAH后脑组织水肿(P<0.05)、NF-κB蛋白表达(P<0.05)、抑制大脑皮质小胶质细胞Iba-1和星形胶质细胞GFAP的活化(P<0.05)。结论柚皮苷在SAH中有明显的抗炎作用。

OBJECTIVE To observe the effects of naringin on neuroinflammation after subarachnoid hemorrhage（SAH） in rats. METHODS Fifty-four male SD rats were randomly divided into sham operation group, SAH group, and naringin group（80 mg·kg^-1）. Rats were anesthetized overdose, after the rats were decapitated, the brain edema was detected by dry-wet weight method. Brain samples from the ipsilateral basal cortex were lysated, centrifuged, extract the protein. NF-κB protein was detected by Western blot. Rats were fixed in 4% paraformaldehyde, and then were embedded in paraffin wax. Iba-1 and GFAP were detected by immunofluorescent staining. RESULTS Compared with SAH group, naringin treatment coould significantly decrease brain edema, the expression of NF-κB protein, and inhibit the activity of GFAP and Iba-1（P〈0.05）. CONCLUSION Naringin has anti-inflammation effect on SAH.