微波辅助法提取核桃青皮中的胡桃醌

Optimization of Microwave-assisted Extraction of Juglone from Walnut Green Husk

研究微波辅助法提取核桃青皮中胡桃醌的最佳工艺条件。以核桃青皮为原料,对料液比、乙醇体积分数、微波时间和微波功率等因素进行研究。在单因素试验基础上,选取料液比、乙醇体积分数和微波时间为考察因素,采用L_9（3~4）正交试验设计,优化核桃青皮中胡桃醌的提取工艺。结果表明,微波功率的影响很小,其它各因子对提取率的影响大小依次是：提取时间〉乙醇体积分数〉料液比;微波辅助法提取的最佳工艺条件：料液比1∶35（g/m L）,乙醇体积分数95%,微波时间25 s。胡桃醌的提取率为1.710 mg/g DW,与预测值1.706 mg/g DW基本一致。

Optimize the microwave-assisted extraction of juglone from walnut green husk.Experimental parameters,such as ratio of solvent to sample,ethanol concentration,extraction time and microwave power,were selected for the single-factor test,then orthogonal experiment design L_9（3~4） was employed to obtain the optimized method.The result showed that factors influencing the extraction of juglone from walnut green husk were ranked as：extraction time〉ethanol concentration〉solvent to sample ratio,and effect of microwave power was considered to be ignored.The optimum extraction method was that solvent to sample ratio 1：35（g/mL）,ethanol concentration 95%,and extraction time 25 s.Using the optimized method,the extraction rate of juglone reached 1.710 mg/g DW,which was consistent with the predictive value of 1.706 mg/g DW.