摘要
以黄秋葵嫩果为原料,通过热水浸提法,乙醇沉淀的工艺获得水溶性黄秋葵多糖,采用DEAE-纤维素离子交换柱纯化多糖,探讨不同pH,不同NaOH浓度和不同NaCl浓度对黄秋葵多糖的洗脱效果,初步确定合适的纯化条件为:以pH 6.0多糖溶液上样,上样量25 m L,先用纯水洗脱,再分别用0.05,0.1,0.3,0.5和0.7 mol/L NaOH进行分步洗脱,洗脱速度为0.47 m L/min,纸层析检验0.05 mol/L NaOH洗脱得较均一多糖。
Water-soluble polysaccharide in Hibiscus esculentus was extracted by means of hot water.Water-soluble polysaccharide in Hibiscus esculentus was departed by means of ethanol sediment,followed by DEAE-cellulose column chromatography.After being studied on different pH values,different kinds of NaOH and different kinds of NaCl concentrations,the purification conditions were found as followings:pH 6.0 polysaccharide solution to the sample,the sample volume 25 mL,first eluting with pure water,then 0.05,0.1,0.3,0.5 and 0.7 mol/L NaOH for elution step respectively,and elution rate 0.47 mL/min,chromatography test determined 0.05 mol/L NaOH elution was more homogeneous polysaccharides.
出处
《食品工业》
CAS
北大核心
2016年第1期84-86,共3页
The Food Industry
基金
浙江省重点科技创新团队项目(2011R50030)
宁波市生态循环农业科技推广项目(2012-7)
关键词
黄秋葵
多糖
DEAE-纤维素
分离纯化
Hibiscus esculentus
polysaccharide
DEAE-cellulose
purification