摘要
目的:建立超高效液相色谱-三重四级杆/线性离子阱质谱(QTRAP-UPLC-MS/MS)同时测定玄参不同产地及商品药材中10种核苷类成分的方法。方法:采用Waters Atlantis T3C18色谱柱(2.1 mm×150mm,3μm),以甲醇-5 mmol·L^-1醋酸铵(含0.1%冰醋酸)为流动相,0.4 m L·min^-1梯度洗脱,正离子多反应监测(MRM)模式测定。结果:10种核苷类成分的浓度与峰面积的线性关系良好(r2〉0.994 7);平均加样回收率为94.72%~98.18%。玄参核苷类成分中,以鸟苷、尿苷、腺苷、尿嘧啶含量较高;不同产地及商品药材中核苷类成分含量有所差异。结论:该方法准确、可靠,为玄参药材内在质量的综合评价提供新的方法参考。
Objective: To establish a QTRAP-UPLC-MS /MS method for simultaneous determination of 10 kinds of nucleosides and nucleobases in Scrophulariae Radix from different habitats and commercial herbs. Methods: The analysis was carried out on an Waters Atlantis T3C18column( 2. 1 mm × 150 mm,3 μm) which was eluted by a mobile phase of methanol-5 mmol·L^-1ammonium acetate( 0. 1% acetic acid) at a flow rate of 0. 4 m L·min^-1. The target compounds were analyzed by the positive ion multiple reaction monitoring( MRM) mode. Results: Ten kinds of nucleosides and nucleobases showed a good linearity( r2〉 0. 994 7) in the range of the tested concentrations. The average recoveries of the ten components were between 94. 72% and 98. 18%. The contents of guanosine,uridine,adenosine and uracil were higher in Scrophulariae Radix. The contents were different from different habitats and commercial herbs. Conclusion: The established method is accurate and reliable,and provides a scientific basis for the quality evaluation of Scrophulariae Radix.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2016年第2期232-237,共6页
Chinese Journal of New Drugs
基金
江苏高校优势学科建设工程资助项目(ysxk-2014)