茄青枯拉尔氏菌Rs-T02在3,4,5-三羟基苯甲酸甲酯作用下的差异蛋白分析

Differential protein analysis of Ralstonia solanacearum strain Rs-T02 after methyl gallate treatment

为探明3,4,5-三羟基苯甲酸甲酯(MG)抑制茄青枯拉尔氏菌的机制,利用二维电泳技术,分析了MG作用下茄青枯拉尔氏菌Rs-T02菌株的差异蛋白。结果发现在MG作用下的菌体中检测出29个差异蛋白点。采用质谱技术鉴定出22个差异蛋白,其中新增蛋白11种、缺失蛋白5种、表达上调蛋白1种和表达下降蛋白5种。这些蛋白参与能量代谢、信号转导、物质运输和DNA修复等途径。我们推测,与能量代谢相关的新增蛋白spot 9和缺失蛋白spot 65可能与MG抑制茄青枯拉尔氏菌的机制有关。

In order to explain the anti-bacterial mechanism of methyl gallate （MG） in Ralstonia solanacearum strain Rs-T02, two-dimensional gel electrophoresis （2-DE） was used to seperate the differential protein of the pathogen with MG treatment. The results showed that 29 differential protein spots were detected. Twenty two proteins, which included 11 newly-emerged, 5 disappeared, 1 up-regulated and 5 down-regulated proteins,were identified by LC-MS/MS. These proteins were related to energy metabolism, signal transduction and transloca- tion of solute or DNA repair. We hypothesized that newly-emerged protein spot 9 and disappeared protein spot 65, which were related to the energy metabolism, might involve in the anti-bacterial mechanism of MG in R. So- lanacearum.