摘要
[目的]探讨孕期尼古丁暴露对子代大鼠脑组织单胺类神经递质水平的影响。[方法]24只SD受孕大鼠,随机分为4组,即对照组和1.0、2.0、4.0 mg/kg染毒组,每组6只。孕7天起,对照组孕鼠皮下注射生理盐水1 m L,染毒组孕鼠皮下注射尼古丁生理盐水溶液1 m L(各染毒组按1.0、2.0、4.0 mg/kg计算尼古丁量),1次/d,直至分娩。测定5周龄子鼠体重、脑重,计算脑脏器系数;测定5周龄子鼠大脑皮质和海马中去甲肾上腺素(NE)、多巴胺(DA)和五羟色胺(5-HT)的质量分数(后简称"含量")。[结果]4.0 mg/kg染毒组子鼠脑重和脑脏器系数低于另3组(P<0.05)。3个染毒组子鼠大脑皮层NE、DA和5-HT含量均低于对照组(P<0.05);4.0 mg/kg染毒组子鼠NE含量低于另2个染毒组(P<0.05),2.0、4.0 mg/kg染毒组子鼠DA含量低于1.0 mg/kg染毒组(P<0.05),4.0 mg/kg染毒组子鼠5-HT含量低于1.0 mg/kg染毒组(P<0.05)。3个染毒组子鼠海马NE和DA含量均低于对照组(P<0.05);海马DA含量与染毒剂量间呈剂量-效应关系(P<0.05),4.0 mg/kg染毒组子鼠海马5-HT含量低于对照组(P<0.05)。[结论]孕期尼古丁暴露影响子代大脑皮层和海马单胺类神经递质含量,可能参与其导致的神经发育毒性过程。
[Objective] To evaluate the effects of gestational exposure to nicotine on the levels of monoamine neurotransmitters in various brain regions of rat offspring. [Methods] Twenty-four pregnant SD rats were randomly divided into four groups and treated with nicotine saline at dose of 0.0, 1.0, 2.0, and 4.0 mg/kg daily through subcutaneous injection starting from the seventh gestational day until delivery. At the age of 5 weeks, the body and brain weights of offspring were measured, brain organ coefficient was calculated, and the levels of norepinephrine (NE), dopamine (DA), and 5-hydroxytryptamine (5-HT) in cerebral cortex and hippocampus of offspring were detected. [Results] The brain weight and brain organ coefficient of the 4.0 mg/kg nicotine treatment group were significantly lower than those of the other three groups (P 〈 0.05). In offspring cerebral cortex, the levels of NE, DA, and 5-HT of the three nicotine exposed groups were significantly lower than those of the control group (P 〈 0.05); the level of NE of the 4.0 mg/kg nicotine treatment group was significantly lower than those of the other two nicotine exposed groups (P 〈0.05); the levels of DA of the 2.0 mg/kg and 4.0 mg/kg nicotine exposed groups were significantly lower than that of the 1.0 mg/kg nicotine exposed group (P 〈 0.05); the level of 5-HT of the 4.0 mg/kg nicotine exposed group was significantly lower than that of the 1.0 mg/kg nicotine exposed group (P 〈 0.05). In offspring hippocampus, the levels of NE and DA of the three nicotine exposed
groups were significantly lower than those of the control group (P 〈 0.05); a dose-effect relationship was found between the DA level and the dose of nicotine treatment (P 〈 0.05); the level of 5-HT in the 4.0 mg/kg nicotine exposed group was significantly lower than those of the control group (P 〈 0.05). [Conclusion] Gestational exposure to nicotine could influence the levels of monoamine neurotransmitters in the cerebral cortex and hippocampus of rat offspring, which might participate the neurodevelopmental toxicity induced by nicotine.
出处
《环境与职业医学》
CAS
CSCD
北大核心
2016年第1期53-56,共4页
Journal of Environmental and Occupational Medicine
关键词
尼古丁
孕期
子代
去甲肾上腺素
多巴胺
五羟色胺
nicotine
gestation
offspring
norepinephrine
dopamine
5-hydroxytryptamine