摘要
对甘蔗属热带种(Saccharum officinarum)越南牛蔗和滇蔗茅(Erianthus rockii Keng)云南95-20杂交后代BC_12个无性系(云南06-278和云南06-279)的根尖进行染色体制片,用以鉴定其体细胞染色体数目和核型分析。结果表明,这2个BC_1后代的体细胞染色体数目均为2n=110,云南06-278和云南06-279核型公式分别为2n=110=116m+4sm和2n=110=102m+8sm,核型分别为2B和2C类型。根据BC_1后代染色体数目鉴定结果,推断杂交F1子代与甘蔗商业品种新台糖10号(ROC10)杂交时,染色体以2n+n方式遗传到BC_1后代。
Two BC1 clones,Yunnan 06-278 and Yunnan 06-279 from intergeneric cross Saccharum officinarum × Erianthus rockii were studied on chromosome karyogram by root tip slice and light microscopy technology. The results showed that both of the two BC1 clones had the same somatic chromosome number 2n = 110. The karyotpye formulae of Yunnan 06-278 and Yunnan 06-279 were respectively as follows： 2n= 110 = 116 m ＋ 4sm as 2B type and 2n = 110 = 102 m ＋ 8sm as 2C type. According to the somatic chromosome number we made inference that when F1 progenies from the cross we studied were back cross to commercial variety ROC10,the chromosomes transfer law from F1 progenies to BC1 progenies probably was 2n ＋ n.
出处
《西南农业学报》
CSCD
北大核心
2015年第6期2413-2420,共8页
Southwest China Journal of Agricultural Sciences
基金
云南省应用基础研究计划青年项目(2012FD071)
国家现代农业产业技术体系甘蔗产业技术体系开远综合试验站(CARS-20-6-13)
农业部2014年农作物种质资源保护项目"甘蔗种质资源收集
编目
更新与利用"(2014NWB017)
科技部
财政部国家科技基础条件平台课题--国家农作物种质资源平台"国家甘蔗种质资源平台"(2014-044)
国家自然科学基金青年基金项目(31501361)
关键词
甘蔗
滇蔗茅
杂交后代
染色体
Sugarcane
Erianthus rockii
Hybrids
Chromosome
