摘要
以姬菇258和金凤2-1为亲本,单孢杂交,获得杂交菌株81株。通过定性出菇表明,其中44号、6号、115号和7号菌株子实体形态具有姬菇的典型特征。通过拮抗反应、ISSR分子标记及聚类分析表明,这4株姬菇新菌株与亲本之间存在遗传差异,证实了杂交姬菇新菌株的真实性;4株新菌株之间也各自保持着个体遗传上的独立和稳定,即构建创制出了4株杂交姬菇新菌株。
81 hybrid strains were obtained by single spore crossbreeding between Pleurotus cornucopiae No. 258 and Pleurotus sp. Jin feng2-1. The fruiting test showed that No. 44,No. 6,No. 115 and No. 7 strains＇ fruiting bodies had the characteristic feature of P. cornucopiae.Antagonistic test,ISSR marker test and cluster analysis showed that the four hybrid strains＇ genetic were different from parents and they were different from each other.
出处
《西南农业学报》
CSCD
北大核心
2015年第6期2655-2660,共6页
Southwest China Journal of Agricultural Sciences
基金
四川省"十二五"农作物及畜禽育种攻关品种选育项目子专题"姬菇新品种选育"(2011NZ0098-7)
国家现代农业产业技术体系四川食用菌创新团队新品种引育研究岗位建设项目(川农业[2009]75号)
四川省财政基因工程专项资金项目"平菇新品种选育"课题
