HIF-1α、E-钙黏蛋白及p53在宫颈癌组织中的表达及临床意义

Expression and clinical significance of HIF-1α, E-cadherin and p53 in cervical carcinoma

目的研究宫颈癌组织中缺氧诱导因子-1α(HIF-1α)、E-钙黏蛋白及p53的表达及其临床意义。方法采用免疫组织化学法检测38例正常宫颈和慢性宫颈炎患者(NNC组)、42例宫颈上皮内瘤变患者(CIN组)、80例浸润性宫颈鳞癌患者(ICC组)宫颈组织中HIF-1α、E-钙黏蛋白及p53的表达。结果 NNC组、CIN组、ICC组患者宫颈组织中HIF-1α表达率分别为10.5%、38.1%、78.8%,E-钙黏蛋白表达率分别为100.0%、71.4%、31.3%,p53阳表达率分别为7.9%、30.1%、61.2%,两两比较差异具有显著性(P<0.05)。HIF-1α和E-钙黏蛋白的表达与临床FIGO分期、组织分化程度和淋巴结是否转移有关。HIF-1α的表达与E-钙黏蛋白表达呈负相关(r=-0.987,P<0.025),与p53表达呈正相关(r=0.452,P<0.028)。结论 HIF-1α/E-钙黏蛋白可能在宫颈癌的进展中发挥重要作用,p53基因的失活可以调节HIF-1α/E-钙黏蛋白通路。HIF-1α、E-钙黏蛋白及p53可以作为宫颈癌诊疗的靶点。

Objective To study expression and clinical significance of hypoxia inducing factor 1-alpha（HIF-1α）, E-cadherin and p53 in cervical carcinoma. Method The expression of HIF-1α, E-cadherin and p53 in normal cervicsl epithelium（NNC group, 38 cases）, intraepithelial neoplasia（CIN group, 42 cases） and cervical carcinoma（ICC group, 80 cases） tissue by immunohistochemistry. Result The expression rate of HIF-1α in cervical tissue of NNC group, CIN group, ICC group were 10.5%, 38.1%, 78.8%, E-cadherin were 100.0%, 71.4%, 31.3% and p53 were 7.9%, 7.9%, 30.1%, compared with the two group had significant difference（P〈0.05）. HIF-1α, E-cadherin and p53 had the relationship with the clinical FIGO staging, whether tissue differentiation degree and lymph node metastasis. The expression of HIF-1 was negatively correlated with the expression of E-cadherin（r = 0.987, P 0.025）, and was positively correlated with the expression of p53（r =0.452, P 0.028）. Conclusion HIF-1α/ E-cadherin may play an important role in the progress of cervical carcinoma. The inactivation of p53 gene can adjust HIF-1α/E-cadherin pathways. HIF-1α, E-cadherin and p53 can as the diagnosis and treatment targets of cervical carcinoma.