摘要
采用离体抑菌测定和盆栽测定相结合的方法,研究了韭菜水提液对甘蓝枯萎病菌1号生理小种的抑制作用和盆栽防治效果。结果表明:韭菜水提液对甘蓝枯萎病菌的菌丝生长速率以及孢子萌发率具有明显的抑制作用,当韭菜水提液浓度为0.75g·m L^(-1)时,菌丝生长抑制率达到100%;当韭菜水提液浓度为0.25g·mL^(-1),处理20h后观察,孢子萌发抑制率达95.47%;在盆栽试验中,基质中加入韭菜水提液浓度为0.086g·g^(-1)接种15d后,甘蓝枯萎病的防治效率可达40%,甘蓝死亡率下降27%。
This paper studied the inhibition role of water extracts from Allium tuberosum on Fusarium oxysporum f.sp.conglutinans(Foc) and control effect of pot cultivation by combining in vitro antibacterial assay with potted plant measurement.The result indicated that extracts could clearly suppress mycelia growth and spore germination.The inhibition rate was up to 100% when the concentration of waterextracts reached 0.75 g · mL^-1.The spore germination inhibition rate reached 95.47% when the added leek extract concentration came to 0.25 g · mL^-1 and was deal with 20 hours.In pot experiments,the disease control efficiency can reach 40%,and mortality fell by 27%15 days after the treatment when the concentration of waterextracts reached 0.086 g · g^-1 in the culture substrate.
出处
《中国蔬菜》
北大核心
2016年第1期60-63,共4页
China Vegetables
基金
国家公益性行业(农业)科研专项(201303014)
现代农业产业技术体系建设专项(CARS-25-B-01)
中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS)
农业部园艺作物生物学与种质创制重点实验室项目
关键词
韭菜
水提液
甘蓝枯萎病菌
盆栽试验
抑制率
Allium tuberosum
Water extract
Fusarium oxysporum f.sp.conglutinans
Potted plant experiment
Inhibition rate